Ordering Information
- : MAB378
- : 100 µL
- : Variable
Product Images
Mouse anti-MAP2 (Catalog Number MAB378) localization of MAP2 in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Western Blot Analysis:
PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-MAP 2 (1:500 dilution of th...
Anti-MAP2A, 2B
Recommended Replacement for: MAB3418| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Am, Av, B, H, M, R, Xn, Qu | IH, WB | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-MAP2A, 2B
Replaces:
MAB3418
Trade Name:
Chemicon (Millipore)
Specificity:
MAP2a & b. Does not recognize other variants.
Molecular Weight:
~ 300 kDa
Immunogen:
Bovine MAP2
Clone:
AP20
Isotype:
IgG1
Background Information:
MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984).
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).
Species Reactivity:
- Amphibian
- Avian
- Bovine
- Human
- Mouse
- Rat
- Xenopus
- Quail
Application Notes:
Immunohistochemistry:
1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.
Western blot:
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
Western blot: A 1:500 dilution was used in a previous lot.
Optimal working dilutions must be determined by end user.
1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.
Western blot:
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
Western blot: A 1:500 dilution was used in a previous lot.
Optimal working dilutions must be determined by end user.
Control:
- Brain tissue
- Cultured neurons
Quality Assurance:
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.
Purification Method:
Unpurified
Presentation:
Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide
Storage Conditions:
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MAP2
- MAP2B
- MAP-2
- MAP2C
- DKFZp686I2148
- MAP2A
Alternate Names:
Microtubule Associated Protein 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
UniProt Summary:
FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
Brand Family:
Chemicon
Product Name:
Anti-MAP2A, 2B
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Ascites
Host:
Mouse