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Référence : MAB381
Qté : 2 mL
Prix Tarif : RMB 3,828.24
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Anti-Myelin Basic Protein, a.a. 119-131, clone 2

Espèces réactives	Principales applications	Hôte	Forme	Type d'anticorps
B, Gp, H, R, Rb, Sh	ELISA, IH	Mouse	Culture Supernatant	Monoclonal Antibody

Code UniProt:

P02686

UniProt Summary

FUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
SIZE: 304 amino acids; 33117 Da
SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family. ... En voir davantage »

Code GenBank:

NM_001025094.1

Entrez Gene Summary

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP ... En voir davantage »

Description:

Anti-Myelin Basic Protein, a.a. 119-131, clone 2

Alternate Names:

MBP

Clone:

Specificity:

Reacts with MBP from human, bovine, rabbit, guinea pig, rat, and sheep. The epitope appears to be located in the region of residues 119-131.

Key Applications:

ELISA
Immunohistochemistry

Application Notes:

Immunohistochemistry - frozen sections

ELISA

Optimal working dilutions must be determined by end user.

Species Reactivity:

Bovine
Guinea Pig
Human
Rat
Rabbit
Sheep

Isotype:

IgG1

Immunogen:

Bovine myelin basic protein.

Epitope:

a.a. 119-131

Presentation:

Tissue culture supernatant. Liquid in 0.2M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.09% sodium azide.

Storage Conditions:

Maintain at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze-thaw cycles.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Gene Symbol:

MBP
MGC99675
Golli-mbp

Antibody Category:

Neuroscience

Antibody Sub-Category:

Neuronal & Glial Markers
Neurochemistry & Neurotrophins

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Trade Name:

Chemicon (Millipore)

Format:

Culture Supernatant

Host:

Mouse

Antibody Type:

Monoclonal Antibody