Guide d'achat
Anti-Myelin Basic Protein, a.a. 82-87
| Espèces réactives | Principales applications | Hôte | Forme | Type d'anticorps |
|---|---|---|---|---|
| B, Ch, Gp, H, M, R, Sh, Rb | ELISA, IC, IH, RIA, WB | Rat | Ascites | Monoclonal Antibody |
Code UniProt:
UniProt Summary
FUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination
...
En voir davantage »
Code GenBank:
Entrez Gene Summary
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related t
...
En voir davantage »
Description:
Anti-Myelin Basic Protein, a.a. 82-87
Background Information:
The two major components of CNS myelin are Myelin Basic Protein (MBP) and proteolipid protein (PLP), occuring in roughly equimolar proportions. There are many neurological disorders associated with deficiencies of myelin assembly and structure. For example Multiple Sclerosis is a human disease due to multiple factors but generally characterized by the degradation of the myelin sheath. MBP ususally refers to the 18.5 kDa isoform, which is one of the most abundant proteins of the myelin sheath of adult human and bovine CNS. Genetically, MBP is the product of differential splicing of a single mRNA transcript. In humans there are 4 isoforms, 21.5, 20.2, 18.5, and 17.2 kDa. Thus, MBP are present in multiple isoforms, part of a large family of developmentally expressed, translocatable, and highly post-translationally modifed proteins with multiple binding partners. Structurally, MBP displays a predominately unorganized form with greater than 75% in random coil. Other non-CNS related functions of myelin include insulin induction and glucagon release from pancreatic islets.
Alternate Names:
MBP
Clone:
12
Specificity:
Reacts with MBP from all species tested. It reacts weakly with peptides ending in the phe 91 where the 91-92 phe-phe bond is broken. Synthetic peptide 82-99 reacts very well as does intact MBP. Mapped to the region DENPVV.
Key Applications:
- ELISA
- Immunocytochemistry
- Immunohistochemistry
- Radioimmunoassay
- Western Blotting
Application Notes:
Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.
ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.
Western Blotting:
Identifies an 18-20kDa band in westerns.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used on frozen sections.
ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.
Western Blotting:
Identifies an 18-20kDa band in westerns.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Bovine
- Chicken
- Guinea Pig
- Human
- Mouse
- Rat
- Sheep
- Rabbit
Species Reactivity Note:
Reacts with MBP from all species tested including human, bovine, sheep, rabbit, mouse, rat, guinea pig and chicken.
Isotype:
IgG2a
Immunogen:
Bovine myelin basic protein
Epitope:
a.a. 82-87
Molecular Weight:
18-20 kDa
Control:
Brain tissue
Presentation:
Liquid containing 0.09% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Gene Symbol:
- MBP
- MGC99675
- Golli-mbp
Quality Assurance:
Evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Neuronal & Glial Markers
- Neurochemistry & Neurotrophins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Asc
Concentration:
variable
Antibody Type:
Monoclonal Antibody
Format:
Ascites
Host:
Rat
Purification Method:
UnPurified