注文に関する情報
Anti-Myelin Proteolipid Protein, C-terminus, clone PLPC1
右記製品の推奨できる代替品: MAB388| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| Ma | IH, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P60201 # This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
SIZE: 277 amino acids; 30,077 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
PTM: Lipoprotein, Palmitate
DISEASE:Defects in PLP1 are the cause of Pelizaeus-Merzbacher disease (PMD) [MIM:312080]. PMD is an X-linked neurologic disorder ... もっと見る »
SIZE: 277 amino acids; 30,077 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
PTM: Lipoprotein, Palmitate
DISEASE:Defects in PLP1 are the cause of Pelizaeus-Merzbacher disease (PMD) [MIM:312080]. PMD is an X-linked neurologic disorder ... もっと見る »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes a transmembrane proteolipid protein that is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5' UTRs, have been identified
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Description:
Anti-Myelin Proteolipid Protein, C-terminus, clone PLPC1
Replaces:
MAB388
Alternate Names:
PLP
Clone:
PLPC1
Specificity:
Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.
Key Applications:
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Species Reactivity:
Mammals
Isotype:
IgG2a
Immunogen:
Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.
Epitope:
C-terminus
Molecular Weight:
23-25 kDa
Control:
Brain
Presentation:
Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- PLP
- PMD
- MMP
- SPG2
- PLP/DM20
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Neuronal & Glial Markers
- Neurochemistry & Neurotrophins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified