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Ordering Information
- : MAB388-100UG
- : 100 μg
- : 1 mg/mL
Anti-Myelin Proteolipid Protein, C-terminus, clone PLPC1
Recommended Replacement for: MAB388| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma | IH, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Myelin Proteolipid Protein, C-terminus, clone PLPC1
Replaces:
MAB388
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.
Molecular Weight:
23-25 kDa
Epitope:
C-terminus
Immunogen:
Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.
Clone:
PLPC1
Isotype:
IgG2a
Species Reactivity:
Mammals
Application Notes:
Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Control:
Brain
Purification Method:
Protein A purfied
Presentation:
Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PLP
- PMD
- MMP
- SPG2
- PLP/DM20
Alternate Names:
PLP
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a transmembrane proteolipid protein that is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5' UTRs, have been identified for this gene.
Key Applications:
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P60201 # This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
SIZE: 277 amino acids; 30,077 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
PTM: Lipoprotein, Palmitate
DISEASE:Defects in PLP1 are the cause of Pelizaeus-Merzbacher disease (PMD) [MIM:312080]. PMD is an X-linked neurologic disorder of myelin metabolism. It is characterized by early impairment of motor development (during the first three months of life) and, later, by the development of abnormal movements and progressive spastic paraplegia.
SIMILARITY: Belongs to the myelin proteolipid protein family.
SIZE: 277 amino acids; 30,077 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
PTM: Lipoprotein, Palmitate
DISEASE:Defects in PLP1 are the cause of Pelizaeus-Merzbacher disease (PMD) [MIM:312080]. PMD is an X-linked neurologic disorder of myelin metabolism. It is characterized by early impairment of motor development (during the first three months of life) and, later, by the development of abnormal movements and progressive spastic paraplegia.
SIMILARITY: Belongs to the myelin proteolipid protein family.
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Myelin Proteolipid Protein, C-terminus, clone PLPC1
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody