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Immunofluorescence staining of human ES cell colonies with anti-SSEA-3 (Catalog Number MAB4303). ES colonies exhibit strong immunoreactivity with SSEA...
Undifferentiated, murine ES cells (cultured in presence of ESGRO®) revealed positive immunostaining for SSEA-1(red) (Catalog Number MAB4301).
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Anti-Stage-Specific Embryonic Antigen-1, clone MC-480
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC, IF, IH, IP | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Stage-Specific Embryonic Antigen-1, clone MC-480
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Molecular Weight:
220 kDa
Immunogen:
F9 tetracarcinoma stem cells (X-irradiated).
Clone:
MC-480
Isotype:
IgM
Background Information:
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Species Reactivity:
- Human
- Mouse
- Rat
Application Notes:
Immunohistochemistry:
A previous lot of this antibody was tested on IH.
Immunoprecipitation:
A previous lot of this antibody was tested on IP.
Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.
Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.
Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.
Optimal working dilutions must be determined by the end user.
A previous lot of this antibody was tested on IH.
Immunoprecipitation:
A previous lot of this antibody was tested on IP.
Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.
Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.
Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.
Optimal working dilutions must be determined by the end user.
Control:
P19 mouse embryonic carcinoma cells
Purification Method:
Ammonium sulfate precipitation
Presentation:
Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- FUT4
- ELFT
- FCT3A
- Fuc-TIV
- 1,3fucosyltransferase
- FUC-TIV
- FucT-IV
- CD15
- Alpha-
- EC 2.4.1.-
Alternate Names:
- SSEA-1
- CD15
- Lewis X
- 3-FAL
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunofluorescence
- Immunohistochemistry
- Immunoprecipitation
Entrez Gene Summary:
FUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.
UniProt Summary:
FUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
SIZE: 405 amino acids; 45570 Da
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.
SIZE: 405 amino acids; 45570 Da
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.
Stem Cell Type:
- Human Embryonic Stem Cells
- Mouse Embryonic Stem Cells
- Neural Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Characterization
Brand Family:
Chemicon
Product Name:
Anti-Stage-Specific Embryonic Antigen-1, clone MC-480
Concentration:
1 .0 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse