Ordering Information

Catalogue Number : MAB4301X
Qty/Pk : 100 μg

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Alternative Format
MAB4301	Anti-Stage-Specific Embryonic Antigen-1, clone MC-480	100 μg

Anti-Stage-Specific Embryonic Antigen-1, clone MC-480, Alexa Fluor® 488 conjugated

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	FC, IC, IF	Mouse	AlexaFluor®488	Monoclonal Antibody

Description:

Trade Name:

Chemicon (Millipore)

Specificity:

Reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Immunogen:

F9 teratocarcinoma stem cells (X-irradiated)

Clone:

MC-480

Isotype:

IgM

Background Information:

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Species Reactivity:

Human
Mouse
Rat

Application Notes:

FACS

Immunocytochemistry:

1. Fix cells with a 4% Paraformaldehyde solution for 30-45 minutes, and then rinse several times with Tris-Buffered-Saline (TBS).

2. Incubate cells with MAB4301X diluted to 1/100 with PBS/ Tween-20 (0.05%) / BSA (1%) for 30 minutes.

3. Wash the sample three times with PBS / Tween-20 (0.05%) for 1 minute per wash. Examine under a microscope fitted with a FITC filter.

Optimal working dilutions must be determined by end user.

Control:

P19 mouse embryonic carcinoma cells

Purification Method:

Ammonium sulfate precipitation

Presentation:

Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide

Storage Conditions:

Maintain refrigerated at 2–8°C protected from light in undiluted aliquots for up to 24 months.

UniProt Number:

P22083

Entrez Gene Number:

NM_002033.2

Gene Symbol:

FUT4
ELFT
FCT3A
Fuc-TIV
1,3fucosyltransferase
FUC-TIV
FucT-IV
CD15
Alpha-
EC 2.4.1.-

Alternate Names:

SSEA-1

Usage Statement:

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications:

Flow Cytometry
Immunocytochemistry
Immunofluorescence

Entrez Gene Summary:

FUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.

UniProt Summary:

FUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
SIZE: 405 amino acids; 45570 Da
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.

Stem Cell Type: