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Ordering Information
- : MAB4303
- : 100 μg
- : 1 mg/mL
Product Images
Brightfield micrograph for comparison of immunofluorescence staining (shown above) of human ES cell colonies with anti-SSEA-3 (Catalog Number MAB4303)...
Anti-Stage-Specific Embryonic Antigen-3, clone MC-631
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ELISA, FC, IF, IH | Rat | Purified | Monoclonal Antibody |
Description:
Anti-Stage-Specific Embryonic Antigen-3, clone MC-631
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells.Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.
Immunogen:
4-8 cell stage mouse embryos
Clone:
MC-631
Isotype:
IgM
Background Information:
The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
Species Reactivity:
- Human
- Mouse
Application Notes:
Immunohistochemistry
Immunofluorescence
FACS Analysis: a starting range of 10-20 μg/mL is suggested.
EIA
Optimal working dilutions must be determined by the end user.
Immunofluorescence
FACS Analysis: a starting range of 10-20 μg/mL is suggested.
EIA
Optimal working dilutions must be determined by the end user.
Control:
Human embryonic stem cells
Purification Method:
Ammonium sulfate precipitation
Presentation:
Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- SSEA-3
- SSEA3
Alternate Names:
SSEA-3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Flow Cytometry
- Immunofluorescence
- Immunohistochemistry
Stem Cell Type:
- Human Embryonic Stem Cells
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Characterization
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Rat
Product Name:
Anti-Stage-Specific Embryonic Antigen-3, clone MC-631
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody