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Ordering Information
- : MAB4304
- : 100 μg
- : 1 mg/mL
Product Images
Immunofluorescence staining of human ES cells with anti-SSEA-4 (Catalog Number MAB4304) monoclonal antibody. As anticipated, ES cells within the colon...
Anti-Stage-Specific Embryonic Antigen-4, clone MC-813-70
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | FC, ELISA, IF, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Stage-Specific Embryonic Antigen-4, clone MC-813-70
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
Immunogen:
Human embryonal carcinoma cell line 2102Ep
Clone:
clone MC-813-70
Isotype:
IgG3
Background Information:
The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
Species Reactivity:
- Human
- Mouse
Application Notes:
Immunohistochemistry: A previous lot of this antibody was used in IH.
Immunofluorescence: A previous lot of this antibody was used in IF.
ELISA: A previous lot of this antibody was used in ELISA.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Immunofluorescence: A previous lot of this antibody was used in IF.
ELISA: A previous lot of this antibody was used in ELISA.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Control:
Pluripotent human embryonic stem (ES) cells
Purification Method:
Protein A purfied
Presentation:
Purified moust monoclonal IgG3 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl, pH 7.6 with 0.1% Sodium Azide
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
- SSEA-4
- SSEA4
Alternate Names:
SSEA-4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- ELISA
- Immunofluorescence
- Immunohistochemistry
Stem Cell Type:
- Human Embryonic Stem Cells
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Characterization
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Stage-Specific Embryonic Antigen-4, clone MC-813-70
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody