订购信息
Anti-TRA-2-49, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E
| 种属反应性 | 关键应用 | 宿主 | 形式 | 抗体类型 |
|---|---|---|---|---|
| H, Po, Rb, Fe, not Gp, not Mk, not M, not R | FC, IF, IC, IP | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-TRA-2-49, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E
Background Information:
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Clone:
clone TRA-2-49/6E
Specificity:
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.
Key Applications:
- Flow Cytometry
- Immunofluorescence
- Immunocytochemistry
- Immunoprecipitation
Application Notes:
Immunocytochemistry: A previous lot was used on IC.
Immunofluorescence: A previous lot of this antibody was used in IF.
FACS Analysis: A starting range of 5-20 µg/mL is suggested.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Immunofluorescence: A previous lot of this antibody was used in IF.
FACS Analysis: A starting range of 5-20 µg/mL is suggested.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Pig
- Rabbit
- Feline
Species Reactivity Note:
Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger. No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.
Non-Reactive Species:
- Guinea Pig
- Monkey
- Mouse
- Rat
Isotype:
IgG1
Immunogen:
2102Ep human Embryonal Carcinoma Cells
Epitope:
Liver/Bone/Kidney Alkaline Phosphatase
Molecular Weight:
212/80/55 kDa.
Control:
Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
Presentation:
Purified mouse monoclonal IgG1 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl pH 7.6 with 0.1% Sodium Azide
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
TRA-2-49
Antibody Category:
Stem Cell Research
Antibody Sub-Category:
Pluripotent & Early Differentiation
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Stem Cell Type:
Human Embryonic Stem Cells
Stem Cell Workflow Stage:
Characterization
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified