Anti-TRA-2-49, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Po, Rb, Fe , not Gp, not M, not Mk, not R | FC, IF, IP | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-TRA-2-49, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E
Background Information:
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Clone:
TRA-2-49/6E
Specificity:
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation.
This antibody does not cross react with other isozymes of AP.
This antibody does not cross react with other isozymes of AP.
Key Applications:
- Flow Cytometry
- Immunofluorescence
- Immunoprecipitation
Application Notes:
This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.
FACS Analysis: a starting range of 5-20 µg/mL is suggested.
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
FACS Analysis: a starting range of 5-20 µg/mL is suggested.
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Human
- Pig
- Rabbit
- Feline
Non-Reactive Species:
- Guinea Pig
- Mouse
- Monkey
- Rat
Isotype:
IgG1
Immunogen:
2102Ep human Embryonal Carcinoma Cells
Epitope:
Liver/Bone/Kidney Alkaline Phosphatase
Molecular Weight:
212/80/55 kDa.
Control:
Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
Presentation:
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
TRA-2-49
Antibody Category:
Stem Cell Research
Antibody Sub-Category:
Pluripotent & Early Differentiation
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
1 mg/mL
Stem Cell Workflow Stage:
Characterization
Stem Cell Type:
Human Embryonic Stem Cells
Format Code:
Pur
Trade Name:
Chemicon (Millipore)
Purification Method:
Protein A Purified
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Mouse