Ordering Information

Catalogue Number : MAB4354
Qty/Pk : 100 µg

Product Images

Flow Cytometry (representative lot data): Flow Cytometry analysis using anti-TRA-2-54. 2102Ep cells were stained with isotype control (grey), or anti...

Anti-TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Mk, Bab, Chp, Pm, not Gp, not M, not Po, not R, not Rb, not Fe	FC, IF, IP	Mouse	Purified	Monoclonal Antibody

Description:

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Trade Name:

Chemicon (Millipore)

Specificity:

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation.

This antibody does not cross react with other isozymes of AP.

Molecular Weight:

212/80/55 kDa.

Epitope:

Liver/Bone/Kidney Alkaline Phosphatase

Immunogen:

2102Ep human Embryonal Carcinoma Cells

Clone:

TRA-2-54/2J

Isotype:

IgG1

Background Information:

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Species Reactivity:

Human
Monkey
Baboon
Chimpanzee
Primate

Species Reactivity Note:

Reactivity has been demonstrated with Liver / Bone / Kidney isozyme of Alkaline Phospatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon) and Old World Monkeys (Baboon, Rhesus Monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey).

No reactivity has been observed with New World Monkeys (Owl Monkey, Squirrel Monkey), Bovine, Canine, Cat, Goat, Guinea Pig, Hamster, Mouse, Pig, Rabbit, Rat Sheep and Tiger.

Non-Reactive Species:

Guinea Pig
Mouse
Pig
Rat
Rabbit
Feline

Application Notes:

This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.

FACS Analysis: a starting range of 5-20 μg/ml is suggested.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Control:

Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Presentation:

Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl pH 7.6 with 0.1% Sodium Azide

Storage Conditions:

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.

Gene Symbol:

TRA-3-54

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications: