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Immunohistochemistry Analysis:
Staining on formalin-fixed, paraffin-embedded human malignant breast carcinoma tissue. Tissue was pretreated with...
Beadlyte ID2 Specificity Assay:
0.5 µg/mL of purified anti-ID2 was incubated with a cocktail of microspheres conjugated to ID1, ID2, ID3, and ID...
Anti-ID2, clone 10C5.2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-ID2, clone 10C5.2
Trade Name:
Chemicon (Millipore)
Molecular Weight:
15 kDa
Immunogen:
Recombinant GST Fusion protein (Human ID2)
Clone:
10C5.2
Isotype:
IgG3κ
Background Information:
ID2 is an inhibitor of differentiation protein, which, along with ID1, ID3, and ID4, plays a key role in the regulation of lineage commitment, cell fate decisions, and in the timing of differentiation during neurogenesis, lymphopoiesis, and neovascularization (angiogenesis). Overexpression of ID2 has been shown to have profound effects on T-cell development and oncogenesis. The ID proteins, induced by the bone morphogenic protein-SMAD pathway, have also been shown to collaborate with LIF to ensure that embryonic stem cells opt for self-renewal.
Species Reactivity:
Human
Presentation:
Purified mouse monoclonal IgG3κ in buffer containing PBS with 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
ID2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. [provided by RefSeq]
Key Applications:
Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: Q02363 # ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
SIZE: 134 amino acids; 14917 Da
SUBUNIT: Heterodimer with other HLH proteins.
SUBCELLULAR LOCATION: Nucleus.
SIZE: 134 amino acids; 14917 Da
SUBUNIT: Heterodimer with other HLH proteins.
SUBCELLULAR LOCATION: Nucleus.
Stem Cell Type:
- Human Embryonic Stem Cells
- Neural Stem Cells
- Epithelial Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Characterization
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse
Product Name:
Anti-ID2, clone 10C5.2
Concentration:
0.41 mg/mL
Antibody Type:
Monoclonal Antibody