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Immunocytochemistry Analysis:
Confocal immunofluorescence analysis of A431 cells using anti-SRF, clone 1E1 (Red). Actin filaments have been label...
Western Blot Analysis:
HeLa nuclear cell lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-SRF, clone 1E1 (0.5 µ...
Anti-SRF [Serum Response Factor], clone 1E1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-SRF [Serum Response Factor], clone 1E1
Specificity:
This monoclonal antibody recognizes SRF.
Molecular Weight:
~52 kDa
Immunogen:
Synthetic human SRF peptide, a.a. 406-508.
Clone:
1E1
Isotype:
IgG1κ
Background Information:
Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE), and is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. SRF is most commonly activated by serum, LPA, LPS, TPA, growth factors, including TNFalpha, as well as antioxidants and UV light.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Reacts with human. A weak interaction with mouse has also been observed. Reactivity with other species has not been tested.
Application Notes:
Immunocytochemistry: Recommended working dilution is 1:500.
Quality Assurance:
(Based upon representative data from a previous lot).
Western Blotting: Recommended working dilution is 0.5 µg/mL.
Western Blotting: Recommended working dilution is 0.5 µg/mL.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ELK3
- ERP
- MCM1
- OTTHUMP00000039820
- SAP2
Alternate Names:
serum response factor (c-fos serum response element-binding transcription factor)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunocytochemistry
UniProt Summary:
FUNCTION: SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentation and maturation.
SUBUNIT STRUCTURE: Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1 By similarity. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1 By similarity.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC.
SEQUENCE SIMILARITIES: Contains 1 MADS-box domain.
SUBUNIT STRUCTURE: Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1 By similarity. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1 By similarity.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC.
SEQUENCE SIMILARITIES: Contains 1 MADS-box domain.
Stem Cell Type:
- Mesenchymal Stem Cells
- Neural Stem Cells
Stem Cell Workflow Stage:
Characterization
Product Name:
Anti-SRF [Serum Response Factor], clone 1E1
Concentration:
1E1
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse