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  • : MAB4401
  • : 100 μg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

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A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect I...

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Anti-Oct-4 Antibody, clone 10H11.2

Species Reactivity Key Applications Host Format Antibody Type
H WB, FC, ICC, ELISA Mouse Purified Monoclonal Antibody
Description:
Anti-Oct-4 Antibody, clone 10H11.2
Promotional Text:
Trade Name:
Chemicon (Millipore)
Product Overview:
Purified monoclonal antibody for the detection of Human Oct-4.
Specificity:
Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
Molecular Weight:
44 kDa
Immunogen:
Recombinant GST fusion protein, human Oct-4 (aa 1-134).
Clone:
clone 10H11.2
Isotype:
Background Information:
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Species Reactivity:
Human
Species Reactivity Note:
The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
Application Notes:
Flow Cytometry: A previous lot of this antibody was used in FC.

ELISA: A previous lot of this antibody was used in ELISA.

Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

Optimal working dilutions must be determined by the end user.
Control:
Human embryonic stem cell lysate.
Quality Assurance:
Evaluated by western blot on mouse P1 brain lysates.

Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates
Purification Method:
Protein A purfied
Presentation:
Purified Ascites presented as a liquid in PBS with 0.1% sodium azide.
Storage Conditions:
Stable for 1 year at from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • POU5F1
  • Oct-3
  • MGC22487
  • Oct4
  • OTF4
  • OTF3
  • OCT3
  • OCT4
  • Oct-4
Alternate Names:
  • Octamer-binding transcription factor 3
  • POU class 5 homeobox 1
  • POU domain class 5, transcription factor 1
  • POU domain, class 5, transcription factor 1
  • POU-type homeodomain-containing DNA-binding protein
  • octamer-binding transcription factor-3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Western Blotting
  • Flow Cytometry
  • Immunocytochemistry
  • ELISA
UniProt Summary:
FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
SIZE: 360 amino acids; 38571 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
Brand Family:
Chemicon
Product Name:
Anti-Oct-4 Antibody, clone 10H11.2
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Applications:
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.

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