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Flow Cytometry Analysis:
C57/BL6 mouse embryonic stem cells were labeled with the Oct-4 antibody, Cat. No. MAB4419, at a 1:200 or 5 µg dilution ...
Immunocytochemistry Analysis:
C57/BL6 mouse embryonic stem cells labeled with DAPI (A) and 1:100 dilution of Oct-4 antibody, Cat. No. MAB4419 (B)...
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Anti-OCT-4 [POU5F1], clone 7F9.2
Recommended Replacement for: MAB4305| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IC, FC, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-OCT-4 [POU5F1], clone 7F9.2
Replaces:
MAB4305
Specificity:
This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.
Molecular Weight:
~39 kDa
Immunogen:
Recombinant human Oct-4 protein, a.a. 1-145.
Clone:
7F9.2
Isotype:
IgG1κ
Background Information:
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Reacts with both human and mouse Oct-4; however, reactivity to other species has not been determined.
Application Notes:
Western Blotting: Recommended working dilution is 1 - 2 µg/mL.
Flow Cytometry: Recommended working dilution is 2 µg/mL.
Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.
Flow Cytometry: Recommended working dilution is 2 µg/mL.
Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.
Purification Method:
Protein G sepharose column
Presentation:
Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- OCT3
- OCT4
- OTF3
- OTF4
- Oct-3
- Oct-4
Alternate Names:
- Octamer-binding transcription factor 3
- POU class 5 homeobox 1
- POU domain class 5, transcription factor 1
- POU-type homeodomain-containing DNA-binding protein
- octamer-binding transcription factor-3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Flow Cytometry
- Western Blotting
UniProt Summary:
FUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
SUBUNIT STRUCTURE:Interacts with UBE2I.
SUBCELLULAR LOCATION: Nucleus. Note= Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICTY: Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.
DEVELOPMENTAL STAGE: Down-regulated during differentiation to endoderm and mesoderm. Ref.1
INDUCTION: Repressed by retinoic acid (RA).
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
SEQUENCE SIMILARITIES: Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
SUBUNIT STRUCTURE:Interacts with UBE2I.
SUBCELLULAR LOCATION: Nucleus. Note= Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICTY: Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.
DEVELOPMENTAL STAGE: Down-regulated during differentiation to endoderm and mesoderm. Ref.1
INDUCTION: Repressed by retinoic acid (RA).
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
SEQUENCE SIMILARITIES: Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Stem Cell Type:
- Human Embryonic Stem Cells
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Characterization
Product Name:
Anti-OCT-4 [POU5F1], clone 7F9.2
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse