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MEL-1 human ES cells labeled with the TG30 antibody, Cat. No. MAB4427 (upper, green) and overlayed with DAPI (lower, blue). Only pluripotent human ES...
TG30 Antibody, clone TG30
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IC, FC, IF | Mouse | Purified | Monoclonal Antibody |
Description:
TG30 Antibody, clone TG30
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody reacts with a cell surface epitope on a 25 kDa protein that was identified as the tetraspannin protein CD9.
Molecular Weight:
25 kDa
Immunogen:
Partially purified preparation of the GCTM-2 antigen.
Clone:
TG30
Background Information:
Human embryonic stem cells (hESCs) have been traditionally characterised using a panel of antibodies to cell surface markers, such as SSEA-3, TRA-1-60 and TRA-1-81; however, none of these traditional markers are entirely specific to hESC cells (Laslett et al., 2003). The novel TG30 antibody, which has been shown to be a reliable cell surface marker for the detection and characterization of pluripotent human embryonic stem cells (Adewumi et al., 2007), recognizes a cell surface epitope of a tetraspannin protein that has been further identified as CD9. When this antibody is used in conjunction with the TG343 antibody, TG30 has been successfully shown through flow cytometric assays to be a robust and rapid tool for the purification and selection of live Oct-4-positive human embryonic stem cells from a mixed population of differentiated cell types.
Species Reactivity:
Human
Species Reactivity Note:
Reacts with human. Reactivity to other species has not yet been determined.
Presentation:
Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
UniProt Number:
Entrez Gene Number:
Alternate Names:
- CD9
- GCTM30
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Flow Cytometry
- Immunofluorescence
Stem Cell Type:
- Human Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Characterization
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Product Name:
TG30 Antibody, clone TG30
Concentration:
2 mg/mL
Antibody Type:
Monoclonal Antibody