Product Images
Mouse anti-Synaptophysin (Catalogue No. MAB5258) localization of synaptophysin in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University o...
Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-synaptophysin (1:100...
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Anti-Synaptophysin, clone SY38)
Recommended Replacement for: CBL203| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B, Av, F | WB, IC, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Synaptophysin, clone SY38)
Replaces:
CBL203
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
Molecular Weight:
38 kDa
Immunogen:
Vesicular fraction of bovine brain
Clone:
clone SY38)
Isotype:
IgG1κ
Background Information:
Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38 kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. The localization and ubiquitous expression of this protein in neuronal tissue makes it an excellent marker for neurons and neuroendocrine tumors. The gene for this protein is located on the X chromosome (Xp11.23-p11.22). The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when the synaptophysin gene is experimentally inactivated in animals, they still develop and function normally.
Species Reactivity:
- Human
- Mouse
- Rat
- Bovine
- Avian
- Fish
Application Notes:
Immunohistochemistry: For relatively cytoplasm rich neuroendocrinic tumors a final concentration of 1 μg/mL is recommended. A final concentration of 2 μg/mL is recommended for cytoplasm deficient tumors.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Control:
Positive Control: Pancreas
Negative Control: Normal mouse serum
Negative Control: Normal mouse serum
Quality Assurance:
Evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this antibody detected synaptophysin on 10 ug of mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this antibody detected synaptophysin on 10 ug of mouse brain lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1κ liquid in buffer containing 20 mM sodium phosphate buffer, 5 mg/mL BSA, with 0.01% methylisothiazolone MIT (w/v), pH 7.5.
Storage Conditions:
Stable for 1 year at from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SYP
- synaptophysin
- Synaptophysin
Alternate Names:
Major synaptic vesicle protein p38
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
Entrez Gene Summary:
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
Brand Family:
Chemicon
Product Name:
Anti-Synaptophysin, clone SY38)
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
50 μg
Format:
Purified
Host:
Mouse