Product Images
Mouse anti-Tyrosine Hydroxylase (Catalogue No. MAB5280) localization of tyrosine hydroxylase in rat midbrain. Photo courtesy of Dr. Robert Sloviter, U...
Western Blot Analysis:
PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Tyrosine Hydroxylase (1:100...
Anti-Tyrosine Hydroxylase, clone 2/40/15
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, Qu, R | IH, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Tyrosine Hydroxylase, clone 2/40/15
Trade Name:
Chemicon (Millipore)
Specificity:
Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.
Molecular Weight:
56-60 kDa
Immunogen:
Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).
Clone:
2/40/15
Isotype:
IgG2a
Background Information:
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Species Reactivity:
- Bovine
- Chicken
- Quail
- Rat
Application Notes:
Immunohistochemistry:
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.
Western blot:
1:1000-1:2000
Optimal working dilutions must be determined by end user.
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.
Western blot:
1:1000-1:2000
Optimal working dilutions must be determined by end user.
Control:
Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue
Quality Assurance:
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Storage Conditions:
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- TH
- TYH
- EC 1.14.16.2
Alternate Names:
TH; Tyrosine Monooxygenase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
UniProt Summary:
FUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Brand Family:
Chemicon
Protein/Isoform Description:
Tyrosine Hydroxylase catalyzes the rate-limiting step in the production of Dopamine.
Product Name:
Anti-Tyrosine Hydroxylase, clone 2/40/15
Concentration:
1 .0 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
40 µg
Format:
Purified
Host:
Mouse