Ordering Information
- : MAB5294-60UL
- : 60 µL
Product Images
Figure 1. Mouse anti-Neurofilament 70 kDa (Catalog Number MAB5294) localization of NF70 in a graft of transplanted fetal porcine ventral mesencephalon...
Figure 2. Mouse anti-Neurofilament 70 kDa (Catalog Number MAB5294) Taken at 10x using dark field optics and shows the same graft as in Figure 1 in gre...
Anti-Neurofilament 70 kDa, Ideal for research on xenograft models, clone DP5 2.7.3
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, Po, not M, not R | IH, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Neurofilament 70 kDa, Ideal for research on xenograft models, clone DP5 2.7.3
Trade Name:
Chemicon (Millipore)
Specificity:
Neurofilament 70 kDa. This antibody recognizes neuroblasts, neurons and peripheral nerves from human, bovine and porcine. It DOES NOT recognize neuroblasts, neurons or peripheral nerves from rodent origin (rat and mouse).
Epitope:
Ideal for research on xenograft models
Immunogen:
Neurofilament purified from bovine spinal cord.
Clone:
DP5 2.7.3
Isotype:
IgG1
Background Information:
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
Species Reactivity:
- Bovine
- Human
- Pig
Non-Reactive Species:
- Mouse
- Rat
Application Notes:
Immunohistochemistry on frozen tissue or paraffin embedded tissue sections: 1:100. The antibody should be diluted in PBS containing 2% BSA, 2% normal horse serum and 0.1% Triton X-100.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Presentation:
Liquid. Immunoglobulin purified from ascites fluid.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- NEFL
- NF68
- CMT2E
- NFL
- CMT1F
- NF-L
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P07196 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
SIZE: 543 amino acids; 61517 Da
DOMAIN: SwissProt: P07196 The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
PTM: O-glycosylated (By similarity).
DISEASE: SwissProt: P07196 # Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). & Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
SIMILARITY: Belongs to the intermediate filament family.
MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
SIZE: 543 amino acids; 61517 Da
DOMAIN: SwissProt: P07196 The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
PTM: O-glycosylated (By similarity).
DISEASE: SwissProt: P07196 # Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years). & Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
SIMILARITY: Belongs to the intermediate filament family.
MISCELLANEOUS: NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
Brand Family:
Chemicon
Product Name:
Anti-Neurofilament 70 kDa, Ideal for research on xenograft models, clone DP5 2.7.3
Antibody Type:
Monoclonal Antibody
Qty/Pk:
60 µL
Format:
Purified
Host:
Mouse