Anti-Notch 1, clone mN1A

P46531

FUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).
SIZE: 2556 amino acids; 272500 Da
SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). & Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleus (By similarity).
TISSUE SPECIFICITY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
DISEASE: SwissProt: P46531 # NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. & Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population.
SIMILARITY: SwissProt: P46531 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 36 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats. ... 隠す » もっと見る »

NM_017617.3

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. ... 隠す » もっと見る »

Anti-Notch 1, clone mN1A

Notch1 consists of three domains. The extracellular domain containing 36 epidermal growth factor (EGF) repeats and 3 lin-12/Notch repeats (LNR) and functions as a receptor in cell-cell interactions. There is a single transmembrane domain (TM). The intracellular domain contains six cdc10/ankyrin repeats (cdc10) and the newly defined Notch cytokine response (NCR) region, which contains a putative bipartite NLS (nuclear localization signal).

mN1A

Recognizes Notch1, intracellular domain. The antibody has a high affinity for the "activated" intracellular form of Notch1, but a low affinity for the full-length (unprocessed or heterodimeric cell surface) forms. No reactivity with Notch2, 3 or 4.

• Immunocytochemistry
• Immunohistochemistry
• Immunoprecipitation
• Western Blotting

Western blot using ECL

Immunohistochemistry

Immunocytochemistry

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

• Mouse
• Rat
• Feline

IgG1

Cdc10-NCR region of mouse Notch1 expressed as a GST fusion protein.

272.5 kDa (also 230 kDa processed form)

In fetal tissues, use spleen, brain stem and lung. In most adult tissues, use lymphoid tissues.

Purified immunoglobulin. Liquid.

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

• NOTCH1
• notch1
• hN1
• TAN1

Epigenetics & Nuclear Function

Transcription Factors

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Chemicon (Millipore)

Pur

1 mg/Ml

Monoclonal Antibody

Purified

Mouse

Protein A Purified