Guide d'achat
Photos des produits
Mouse anti-NG2 (Catalog Number MAB5384). Localization of NG2 in adult rat cortex.
Anti-NG2 Chondroitin Sulfate Proteoglycan
| Espèces réactives | Principales applications | Hôte | Forme | Type d'anticorps |
|---|---|---|---|---|
| H, M, R | WB, IC, ELISA, IH | Mouse | Purified | Monoclonal Antibody |
Code UniProt:
UniProt Summary
FUNCTION: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependen
...
En voir davantage »
Code GenBank:
Entrez Gene Summary
extracellular matrix protein secreted by many cell types; member of the large chondroitin sulfate proteoglycan (CSPG) family; may be involved with angiogenesis -dependent processes such as wound healing or tumor growth [RGD]
Description:
Anti-NG2 Chondroitin Sulfate Proteoglycan
Background Information:
NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
Clone:
132.39
Specificity:
NG2 Chondroitin Sulfate Proteoglycan. MAB5384 reacts with native, recombinant and protein from cells which express NG2.
Key Applications:
- Western Blotting
- Immunocytochemistry
- ELISA
- Immunohistochemistry
Application Notes:
Immunocytochemistry: 1:200-1:500 dilution of a previous lot was used on oligodendrocyte precursor cells.
ELISA (direct): A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
ELISA (direct): A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Rat. Expected to react with human and mouse based on sequence similarity.
Isotype:
IgG1
Immunogen:
Cell line expressing a truncated form of NG2.
Molecular Weight:
~ 280 kDa
Control:
Neonatal rat brain extract
Presentation:
Liquid in 0.02 M Phosphate buffer, containing 0.25 M NaCl and 0.1% azide.
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
- CSPG4
- MSK16
- MCSP
- NG2
- MCSPG
- MEL-CSPG
- HMW-MAA
Quality Assurance:
Evaluated by Western Blot on rat brain lysates.
Western Blot Analysis: 1:500 dilution of this antibody detected NG2 Chondroitin Sulfate Proteoglycan on 10 µg of rat brain lysates.
Western Blot Analysis: 1:500 dilution of this antibody detected NG2 Chondroitin Sulfate Proteoglycan on 10 µg of rat brain lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G Purified