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Ordering Information
- : MAB5790
- : 100 μg
- : See Certificate of Analysis
Product Images
Western Blot Analysis:
Cat. # MAB5790 can detect DSD-1 at 0.1 µg/mL in mouse brain tissue Lysate.
Anti-DSD-1, clone 473HD
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, Ch, Am | WB, IP, IC, ELISA | Rat | Purified | Monoclonal Antibody |
Description:
Anti-DSD-1, clone 473HD
Trade Name:
Chemicon (Millipore)
Specificity:
Cat. # MAB5790 recognizes the DSD-1 glycosaminoglycan structure. The DSD-1 epitope is part of DSD-1 proteoglycan, the mouse homologue of rat phosphacan.
Molecular Weight:
Smear between 400-1000 kDa.
Epitope:
DSD-1
Immunogen:
"rest-L2" glycoprotein fraction from adult mouse brain
Clone:
473HD
Isotype:
IgMκ
Background Information:
Anti-DSD-1 reacts with a glycosaminoglycan epitope selectively expressed on radial gliacytes of which a subpopulation shows stem cell properties. Cells isolated with this antibody from embryonic mouse tissue had multipotent characteristics and could turn into neurons, astrocytes and oligodendrocytes. The epitope is highly conserved and has been found in several vertebrates.
Species Reactivity:
- Mouse
- Human
- Chicken
- Amphibian
Application Notes:
Western Blot Analysis:
Cat. # MAB5790 can detect DSD-1 at 0.1 µg/mL in mouse brain tissue Lysate.
Immunocytochemistry: 1 µg/mL on frozen tissue sections with 4%w/v paraformaldehyde in PBS (6,8).
Immunoprecipitation: 10 µg/mL as working concentration (9).
ELISA: Direct ELISA with working concentration of 1 µg/mL or less (2).
Cat. # MAB5790 can detect DSD-1 at 0.1 µg/mL in mouse brain tissue Lysate.
Immunocytochemistry: 1 µg/mL on frozen tissue sections with 4%w/v paraformaldehyde in PBS (6,8).
Immunoprecipitation: 10 µg/mL as working concentration (9).
ELISA: Direct ELISA with working concentration of 1 µg/mL or less (2).
Control:
mouse brain lysate
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Purified immunoglobulin
Presentation:
Purified in PBS with 0.05% NaN3
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
UniProt Number:
Gene Symbol:
PTPRB
Alternate Names:
- DSD-1
- DSD-1-PG
- DSD-1 proteoglycan
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
- Immunocytochemistry
- ELISA
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Rat
Product Name:
Anti-DSD-1, clone 473HD
Concentration:
See Certificate of Analysis
Antibody Type:
Monoclonal Antibody