Ordering Information
- : MAB88051
- : 50 µg
- : 0.2 mg/mL
Anti-Heat Shock Protein 27, clone G3.1
Recommended Replacement for: MAB3512| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk, Chp, not M, not R | ELISA, IF, WB, IH, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Heat Shock Protein 27, clone G3.1
Replaces:
MAB3512
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes an estrogen-regulated protein of 24-27 kDa which is identified as hsp27. Hsp27 was recently found to be identical to the estrogen induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. Hsp27 has also been implicated in drug resistance in cancer cells.
CELLULAR LOCALIZATION:
Cytoplasmic / nuclear.
CELLULAR LOCALIZATION:
Cytoplasmic / nuclear.
Immunogen:
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Clone:
G3.1
Isotype:
IgG1
Background Information:
ANTIGEN DISTRIBUTION:
Hsp27 is identical to estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are hsp27 positive, especially those that are positive for ER and/or PgR. Hsp 27 is also complicated in drug resistance. MAB88051 is excellent for biochemical and histochemical applications.
Hsp27 is identical to estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are hsp27 positive, especially those that are positive for ER and/or PgR. Hsp 27 is also complicated in drug resistance. MAB88051 is excellent for biochemical and histochemical applications.
Species Reactivity:
- Human
- Monkey
- Chimpanzee
Non-Reactive Species:
- Mouse
- Rat
Application Notes:
Immunohistochemistry (frozen & formalin/paraffin): 1-2 μg/mL. 30-60 minutes at room temperature. Staining of
formalin fixed tissue sections requires boiling the tissue
sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes
followed by cooling at room temperature for 20 minutes. Both nuclear and cytosolic staining will be observed.
Western blot: 1-2 μg/mL. 2hrs RT; simple buffers without milk as cross reactivity with bovine HSP's may occur.
Immunofluorescence: 4% PFA fixed tissues; permabilize with 0.1% triton X-100 in block: 1:50-1:200.
ELISA (capture antibody; use goat anti-mouse Fc coating antibody, then coat with G3.1, 0.1-2μg/mL).
Optimal working dilutions must be determined by end user.
formalin fixed tissue sections requires boiling the tissue
sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes
followed by cooling at room temperature for 20 minutes. Both nuclear and cytosolic staining will be observed.
Western blot: 1-2 μg/mL. 2hrs RT; simple buffers without milk as cross reactivity with bovine HSP's may occur.
Immunofluorescence: 4% PFA fixed tissues; permabilize with 0.1% triton X-100 in block: 1:50-1:200.
ELISA (capture antibody; use goat anti-mouse Fc coating antibody, then coat with G3.1, 0.1-2μg/mL).
Optimal working dilutions must be determined by end user.
Control:
POSITIVE CONTROL: BT474 or MDA-MB-453 cells and breast carcinoma.
Presentation:
Purified immunoglobulin presented as a liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Storage Conditions:
Maintain material at 2-8°C for up to 12 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- HSPB1
- Hs.76067
- HSP27
- Hsp25
- HspB1
- HSP28
- CMT2F
- HS.76067
- DKFZp586P1322
- HMN2B
- SRP27
Alternate Names:
HSP27
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunofluorescence
- Western Blotting
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.
SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.
Brand Family:
Chemicon
Product Name:
Anti-Heat Shock Protein 27, clone G3.1
Concentration:
0.2 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
50 µg
Format:
Purified
Host:
Mouse