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Ordering Information
- : MAB8878
- : 50 µg
- : 0.2 mg/mL
Anti-Cdk1, clone A17.1.1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, R, Xn | IF, IP, EA, WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Cdk1, clone A17.1.1
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes a 34 kDa protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks).
Immunogen:
Raised against Xenopus p34cdc2.
Clone:
A17.1.1
Isotype:
IgG2a
Species Reactivity:
- Chicken
- Human
- Mouse
- Rat
- Xenopus
Application Notes:
Frozen Sections
Immunofluorescence
Immunoprecipitation: (Native/denatured, Protein A; 2μg/mg protein lysate)
Immunohistology (Formalin, 2-4μg/mL for 30 min at RT)
Paraffin Sections (staining requires antigen retrieval).
Western Blotting
Kinase Assay
Optimal working dilutions must be determined by the end user.
p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. MAB8878 inhibits the activation of p34cdc2 kinase by cyclins and precipitates an active kinase.
Immunofluorescence
Immunoprecipitation: (Native/denatured, Protein A; 2μg/mg protein lysate)
Immunohistology (Formalin, 2-4μg/mL for 30 min at RT)
Paraffin Sections (staining requires antigen retrieval).
Western Blotting
Kinase Assay
Optimal working dilutions must be determined by the end user.
p34cdc2, a serine/threonine kinase, is activated by cyclins, presumably by dephosphorylation of tyrosine residues. MAB8878 inhibits the activation of p34cdc2 kinase by cyclins and precipitates an active kinase.
Control:
POSITIVE CONTROL:
HeLa or NIH3T3 cells. Germinal center in a tonsil or reactive lymph node. Cellular localization is predominantly nuclear, with some cytoplasmic staining.
HeLa or NIH3T3 cells. Germinal center in a tonsil or reactive lymph node. Cellular localization is predominantly nuclear, with some cytoplasmic staining.
Presentation:
Purified immunoglobulin. 0.2 mg/mL in 10 mM PBS, pH 7.4, containing 0.2% BSA and 0.09% Sodium Azide.
Storage Conditions:
Maintain at 2° to 8°C in undiluted aliquots for up to 12 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CDC2
- CDK1
- DKFZp686L20222
- MGC111195
- CDC28A
- EC 2.7.11.22
- EC 2.7.11.23
Alternate Names:
p34cdc2; CDC2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
Key Applications:
- Immunofluorescence
- Immunoprecipitation
- Enzyme Assay
- Western Blotting
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
50 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Cdk1, clone A17.1.1
Concentration:
0.2 mg/mL
Antibody Type:
Monoclonal Antibody