Ordering Information
- : MABN42
- : 100 μg
- : 0.5 mg/mL
Product Images
Western Blotting Analysis:
Representative lot data.
Mouse brain microsomal tissue lysate was probed with Anti-Neuregulin-1, clone D11 (0.2...
Anti-Neuregulin-1, clone D11
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, ICC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Neuregulin-1 Antibody, clone D11 | MABN42
Promotional Text:
Specificity:
This antibody recognizes Neuregulin-1 at the extracellular domain.
Molecular Weight:
~29 kDa observed. This target has been shown to undergo differential splicing during development into ~30 different isoforms.
Epitope:
Extracellular domain
Immunogen:
His-tagged recombinant protein corresponding to mouse Neuregulin-1 at the extracellular domain.
Clone:
D11
Isotype:
IgG1κ
Background Information:
The group of Neuregulin-1 proteins are cell-cell signaling molecules and are ligands for receptor tyrosine kinases of the ErbB/HER subfamily. Type III NRG1 proteins (also referred to as CRD-NRG1 proteins) play a critical role in neural development, including myelination, motor and sensory neuron survival, and neuromuscular synapse development (Falls, 2003). Defects in animals lacking type III NRGs include retraction of nerve terminals from newly formed synapses, absence of Schwann cells from peripheral nerves, and loss of motor and sensory neurons (Wolpowitz et al., 2000). Available evidence indicates that most Type III NRG1 proteins are synthesized as transmembrane proteins with 2 TM domains, and that proteolytic processing produces a bioactive transmembrane form (NTFm) which serves as a juxtacrine signal (Wang, 2001; Falls, 2003).
Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with mouse.
Application Notes:
Immunocytochemistry Analysis: A previous lot of this antibody was used successfully by an independent laboratory in IC. (Pirotte, D., et al.)
Control:
Mouse brain microsomal tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain microsomal tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Neuregulin-1 on 10 µg of mouse brain microsomal tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected Neuregulin-1 on 10 µg of mouse brain microsomal tissue lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- GGF
- NDF
- GGF2
- HGL
- MST131
- HRGA
- HRG
- HRG1
- SMDF
- ARIA
- pro-NRG1
- MSTP131
- Pro-NRG1
Alternate Names:
- neuregulin 1
- sensory and motor neuron derived factor
- neu differentiation factor
- pro-neuregulin-1, membrane-bound isoform
- heregulin, alpha (45kD, ERBB2 p185-activator)
- heregulin, alpha (45kD, ERBB2 p185-activator)
- glial growth factor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunocytochemistry
Product Name:
Anti-Neuregulin-1, clone D11
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse