Ordering Information
- : MABS62
- : 100 μg
- : 0.5 mg/mL
Product Images
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded prostate tissue was prepared using heat-induced epitope retrieva...
Western Blotting Analysis:
Representative lot data.
MCF-7 cells treated with 1 nM Estradiol for 72 hrs was probed with Anti-GREB1, clone G...
Anti-GREB1, clone GREB1Ab
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, ICC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-GREB1 Antibody, clone GREB1Ab | MABS62
Promotional Text:
Molecular Weight:
~ 216 kDa observed. Uncharacterized bands may be observed in some lysates.
Immunogen:
Recombinant protein corresponding to human GREB1.
Clone:
GREB1Ab
Isotype:
IgG1κ
Background Information:
GREB1, also known as gene regulated in breast cancer protein 1 is shown to be involved in the proliferation of breast cancer cells, controlled by estrogen. GREB1 has the potential to be a clinical marker for response to endocrine therapy and a therapeutic target. GREB1 also may be the regulator for prostate cancer.
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Application Notes:
Immunohistochemistry Analysis: 1:600 dilution from a previous lot detected GREB1 in prostate tissue.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IP. (Hnatyszyn, H., et al. 2009). Breast Cancer Res Treat
DOI 10.1007/s10549-009-0584-x.)
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IP. (Hnatyszyn, H., et al. 2009). Breast Cancer Res Treat
DOI 10.1007/s10549-009-0584-x.)
Control:
MCF-7 cell lysate
Quality Assurance:
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected GREB1 on 10 µg of MCF-7 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected GREB1 on 10 µg of MCF-7 cell lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
GREB1
Alternate Names:
- growth regulation by estrogen in breast cancer 1
- Gene regulated in breast cancer 1 protein
- protein GREB1
- gene regulated by estrogen in breast cancer protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunohistochemistry
- Immunocytochemistry
Entrez Gene Summary:
This gene is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq].
UniProt Summary:
FUNCTION: Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
TISSUE SPECIFICTY: Expressed in proliferating prostatic tissue and prostate cancer.
INDUCTION: Estrogen-responsive; regulated by estrogen receptors. Regulated by androgens. The regulatory region of the gene contains 3 estrogen-responsive elements.
SEQUENCE SIMILARITIES: Belongs to the GREB1 family.
SEQUENCE CAUTION: The sequence BAA25501.2 differs from that shown. Reason: Erroneous initiation.
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
TISSUE SPECIFICTY: Expressed in proliferating prostatic tissue and prostate cancer.
INDUCTION: Estrogen-responsive; regulated by estrogen receptors. Regulated by androgens. The regulatory region of the gene contains 3 estrogen-responsive elements.
SEQUENCE SIMILARITIES: Belongs to the GREB1 family.
SEQUENCE CAUTION: The sequence BAA25501.2 differs from that shown. Reason: Erroneous initiation.
Product Name:
Anti-GREB1, clone GREB1Ab
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse