Ordering Information
- : MCYTOMAG-70K-PMX
- : 96-well plate
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MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex
Description:
MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex
Trade Name:
MILLIPLEX
Qty/Pk:
96-well plate
Product Overview:
To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing
MILLIPLEX MAP Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing
MILLIPLEX MAP Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background Information:
The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.
Key Applications:
Multiplexing
Application Notes:
• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
• This assay requires 25 µl of 1:2 diluted plasma or serum, or 25 μL cell culture supernatant per well.
• This assay requires 25 µl of 1:2 diluted plasma or serum, or 25 μL cell culture supernatant per well.
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Configuration:
Premixed
Sensitivity:
Refer to kit protocol for sensitivities for individual cytokines.
Premixed or Single Plex:
Yes
Panel Type:
Cytokines/Chemokines - Magnetic
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Precision, %:
- Inter-assay: 4 - 12
- Intra-assay: 1 - 5
Accuracy:
85% - 107%
Detection Methods:
Luminex xMAP
Packaging:
96 well plate
Cross Reactivity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Product Name:
MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex
Standard Curve Range:
3.2 – 10,000 pg/mL
Assay Panel:
Premixed beads for your convenience.
Analytes Available:
G-CSF, GM-CSF, IFN-γ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IP-10, KC-like, MCP-1, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α