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Typical Standard Curve for EpiQuant Phosphotyrosine Cell Signaling Magnetic Bead Panel 2
MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Magnetic Bead Panel 2 - Cell Signaling Multiplex Assay
| Number of Analytes Available | Research Areas | Detection Methods |
|---|---|---|
| 21 | Oncology | Luminex xMAP |
Description:
MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Magnetic Bead Panel 2, MPEQMAG-102K
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Background Information:
The MILLIPLEX MAP EpiQuant Phosphotyrosine Cell Signaling Magnetic Bead Panel 2 is a Luminex based approach for assessing the functional states of cells by profiling tyrosine phosphorylation changes across signaling pathways and cell phenotypes. Site-specific, quantitative measurements with picomolar sensitivity support diverse sample types and applications across the drug discovery / development continuum.
Key Applications:
Multiplexing
Application Notes:
• This is a two-hour room temperature incubation assay.
• This assay requires 25 µL prepared cell culture lysate.
• ONLY SAMPLES PREPARED USING THE EPIQUANT SAMPLE PREPARATION KIT (MPEQ-SP) WILL GENERATE RELIABLE RESULTS WITH THIS ASSAY.
• This assay requires 25 µL prepared cell culture lysate.
• ONLY SAMPLES PREPARED USING THE EPIQUANT SAMPLE PREPARATION KIT (MPEQ-SP) WILL GENERATE RELIABLE RESULTS WITH THIS ASSAY.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sensitivity:
Refer to kit protocol for sensitivities
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Modifications:
Phosphorylation
Cross Reactivity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Standard Curve Range:
-
0.64 – 2,000 pM: AXL (Tyr772), AXL (Tyr859)
EpoR (Tyr426), GSK3α/β (Tyr216/279), Heterogeneous nuclear ribonucleoprotein K (Tyr72), Rai-like protein (Tyr424), Rai-like protein (Tyr465), and Vanilloid receptor-like channel 2 (Tyr411), Vav1 (Tyr142/160/174), VEGFR3 (Tyr1230/1231) - 3.2 – 10,000 pM: BTK (Tyr344), GHR (Tyr487), HGFR/met (Tyr1365), Kit/SCFR (Tyr545), Kit/SCFR (Tyr936), LSR (Tyr406/407, LSR (Tyr615/616), PSTPIP1 (Tyr344), RET (Tyr1062/1090/1096), RET (Tyr826), Ron/MSP-R (Tyr1353/1360), SYK (Tyr629/630)
Product Name:
MILLIPLEX® MAP EpiQuant™ Phosphotyrosine Cell Signaling Magnetic Bead Panel 2, MPEQMAG-102K
Materials Required but Not Delivered:
MILLIPLEX® MAP EpiQuant™ Sample Preparation Kit (MPEQ-SP)
Analytes Available:
AXL (Tyr772), AXL (Tyr859), BTK (Tyr344), EpoR (Tyr426), GHR (Tyr487), HGFR/met (Tyr1365), Heterogeneous nuclear ribonucleoprotein K (Tyr72), Kit/SCFR (Tyr545), Kit/SCFR (Tyr936), LSR (Tyr406/407), LSR (Tyr615/616), PSTPIP1 (Tyr344), RET (Tyr1062/1090/1096), RET (Tyr826), Rai-like protein (Tyr424), Rai-like protein (Tyr465), Ron/MSP-R (Tyr1353/1360), SYK (Tyr629/630), VEGFR3 (Tyr1230/1231), Vanilloid receptor-like channel 2 (Tyr411), Vav1 (Tyr142/160/174)
Configuration:
Kit
Panel Type:
Cell Signaling
Precision, %:
- Intra-Assay < 5%
- Inter-Assay < 10% - 20%
Accuracy:
92% - 104%
Detection Methods:
Luminex xMAP
Packaging:
96 well plate
Entrez Gene Number:
Product Resources
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