Ordering Information
- : NC011
- : 50 µg
- : 1 mg/mL
Nerve Growth Factor 2.5s, mouse submaxillary glands
Description:
Nerve Growth Factor 2.5s, mouse submaxillary glands
Trade Name:
Chemicon (Millipore)
Qty/Pk:
50 µg
Product Overview:
MOLECULAR WT.:
13,000 reduced
13,000 reduced
Background Information:
Murine 2.5S Nerve Growth Factor is a 26kDa protein composed of two identical 118 amino acid chains under native conditions.. The 2.5S form of murine Nerve Growth Factor originates from the dissociation and proteolytic cleavage of the beta subunit of 7S forms of this neurotrophic factor and is also referred to as beta-NGF. This form mediates phosphorylation of specific intracellular proteins and is solely responsible for the biological activity of both the 2.5S and 7S forms of this neurotrophic factor. Murine 2.5S Nerve Growth Factor is expressed in sympathetic and sensory-innervated peripheral tissues such as the vas deferens, heart, iris, skin, splenic capsule, sciatic nerve and submaxillary gland. At the cellular level, mNGF expression has been demonstrated in lymphocytes, smooth muscle cells, epithelial cells, astrocytes, fibroblasts and Schwann cells. There is a strong correlation between the levels of mNGF mRNA in peripheral tissues and the density of sympathetic innervation. Peripheral neurons that respond to mNGF include adrenergic sympathetic neurons and primary sensory neurons. Cellular responses to this neurotrophic factor include differentiation, neurotic extension and production of catecholamine and neuropeptide-synthesizing enzymes. Murine Nerve Growth Factor binds the "low affinity" NGF receptor (p75NTR), as do all neurotrophic factors, and it mediates, via p75NTR signal transduction, the functional response of Schwann cells. mNGF also activates signal transduction by the dimerization and autophosphorylation of the TrkA receptor (also known as Trk and p140trk).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Mouse
Brand Family:
Chemicon
Source:
Mouse submaxillary gland.
Presentation:
Lyophilized. Reconstitute to a concentration of 10 μg/mL with sterile DMEM. Medium with mNGF cannot be reused due to mNGF degradation.
UniProt Number:
Specific Activity:
BIOLOGICAL ASSAY:
This lot was bioassayed for neurotrophic activity using rat PC12 cell cultures over a period of 7-14 days. The activity is in the range of 0.5-50 ng/mL. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50 ng/mL of medium (Bradshaw, 1978).
This lot was bioassayed for neurotrophic activity using rat PC12 cell cultures over a period of 7-14 days. The activity is in the range of 0.5-50 ng/mL. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50 ng/mL of medium (Bradshaw, 1978).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain frozen at -70°C in undiluted aliquots for 4-6 weeks. Avoid repeated freeze-thaw cycles as it may result in a loss of activity.
Packaging:
2x25 μg
Gene Symbol:
- NGF
- NGFB
Protein or Enzyme Type:
Neurobiology
Product Name:
Nerve Growth Factor 2.5s, mouse submaxillary glands
Alternate Names:
beta-NGF
Concentration:
1 mg/mL
Purity:
Greater than 90% determined by SDS-PAGE electrophoresis.