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Ordering Information
- : NS140
- : 1 kit
Embryonic Stem Cell Derived Neuron Integration and Characterization Kit
Description:
Embryonic Stem Cell Derived Neuron Integration and Characterization Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Stem cell research and applications are rapidly moving toward the introduction and subsequent post-development analysis of embryonic stem (ES) cell-derived neurons in adult tissue. The NS140 is part of an expanding Chemicon product line targeting development and plasticity in neurobiology. NS140 is a novel, broadly encompassing kit for the localization, characterization and analysis of ES cell derived neuron integration into adult central nervous system (CNS) tissue. This kit allows researchers to: (1) classify neuronal pharmacological subtype and (2) identify and quantify excitatory and inhibitory synapses involving the transplanted cells. The kit component breakdown is as follows:
(1) Classify neuronal pharmacological subtype. Researchers are interested in identifying the subtype of neuron that their tagged ES have matured into following introduction and integration into the recipient CNS. The kit includes antibodies for the identification of Glutamate neurons (EAAC1; AB1520-25UL), GABAergic neurons (GAD67; MAB5406-50UG), Dopaminergic neurons (TH; MAB5280-20UG), and serotonergic neurons (Serotonin; MAB352-50UL).
(2) Identify and quantify excitatory and inhibitory synapses involving the transplanted cells. In order to measure the level of functional integration of the tagged ES cells, researchers often use general markers of mature synaptic formation and identification of inhibitory GABAergic synaptic input onto the donor cells. The kit includes antibodies for the general identification of synapses (PSD-95; MAB1596-50UG) and (Synaptophysin; MAB5258-20UG) as well as for GABAergic synapses (GABAA Receptor b-chain; MAB341-50UG).
(1) Classify neuronal pharmacological subtype. Researchers are interested in identifying the subtype of neuron that their tagged ES have matured into following introduction and integration into the recipient CNS. The kit includes antibodies for the identification of Glutamate neurons (EAAC1; AB1520-25UL), GABAergic neurons (GAD67; MAB5406-50UG), Dopaminergic neurons (TH; MAB5280-20UG), and serotonergic neurons (Serotonin; MAB352-50UL).
(2) Identify and quantify excitatory and inhibitory synapses involving the transplanted cells. In order to measure the level of functional integration of the tagged ES cells, researchers often use general markers of mature synaptic formation and identification of inhibitory GABAergic synaptic input onto the donor cells. The kit includes antibodies for the general identification of synapses (PSD-95; MAB1596-50UG) and (Synaptophysin; MAB5258-20UG) as well as for GABAergic synapses (GABAA Receptor b-chain; MAB341-50UG).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
UniProt Summary:
FUNCTION: SwissProt: Q99259 # Catalyzes the production of GABA.
COFACTOR: Pyridoxal phosphate.
SIZE: 594 amino acids; 66897 Da
SUBUNIT: Homodimer.
TISSUE SPECIFICITY: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
DISEASE: SwissProt: Q99259 # Defects in GAD1 are the cause of autosomal recessive symmetric spastic cerebral palsy (SCP) [MIM:603513]. Cerebral palsy (CP) is an heterogeneous group of neurological disorders of movement and/or posture, with an estimated incidence of 1 in 250 to 1'000 live births, making CP one the commonest congenital disabilities. Non-progressive forms of symmetrical, spastic CP have been identified, which show a Mendelian autosomal recessive pattern of inheritance. Patients present developmental delay, mental retardation and sometimes epilepsy as part of the phenotype.
SIMILARITY: SwissProt: Q99259 ## Belongs to the group II decarboxylase family.
COFACTOR: Pyridoxal phosphate.
SIZE: 594 amino acids; 66897 Da
SUBUNIT: Homodimer.
TISSUE SPECIFICITY: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
DISEASE: SwissProt: Q99259 # Defects in GAD1 are the cause of autosomal recessive symmetric spastic cerebral palsy (SCP) [MIM:603513]. Cerebral palsy (CP) is an heterogeneous group of neurological disorders of movement and/or posture, with an estimated incidence of 1 in 250 to 1'000 live births, making CP one the commonest congenital disabilities. Non-progressive forms of symmetrical, spastic CP have been identified, which show a Mendelian autosomal recessive pattern of inheritance. Patients present developmental delay, mental retardation and sometimes epilepsy as part of the phenotype.
SIMILARITY: SwissProt: Q99259 ## Belongs to the group II decarboxylase family.
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Characterization
Components:
- 50 μg of GABAA Receptor β-chain monoclonal antibody MAB341-50UG
- 50 μg of GAD67 monoclonal antibody MAB5406-50UG
- 50 μg of PSD-95 monoclonal antibody MAB1596-50UG
- 50 μL of Serotonin monoclonal antibody MAB352-50UL
- 20 μg of Synaptophysin monoclonal antibody MAB5258-20UG
- 20 μg of Tyrosine Hydroxylase monoclonal antibody MAB5280-20UG
- 25 μL of Glutamate Transporter, Neuronal (EAAC1) polyclonal antibody AB1520-25UL
Brand Family:
Chemicon
UniProt Number:
Storage Conditions:
Maintain components according to the individual data sheets.
Gene Symbol:
- GABRB2
- MGC119386
- GABA
- MGC119389
- MGC119388
Product Name:
Embryonic Stem Cell Derived Neuron Integration and Characterization Kit
Entrez Gene Number: