Embryonic Stem Cell Derived Neuron Integration and Characterization Kit
Description:
Embryonic Stem Cell Derived Neuron Integration and Characterization Kit
Trade Name:
Chemicon (Millipore)
Product Overview:
Stem cell research and applications are rapidly moving toward the introduction and subsequent post-development analysis of embryonic stem (ES) cell-derived neurons in adult tissue. The NS140 is part of an expanding Chemicon product line targeting development and plasticity in neurobiology. NS140 is a novel, broadly encompassing kit for the localization, characterization and analysis of ES cell derived neuron integration into adult central nervous system (CNS) tissue. This kit allows researchers to: (1) classify neuronal pharmacological subtype and (2) identify and quantify excitatory and inhibitory synapses involving the transplanted cells. The kit component breakdown is as follows:
(1) Classify neuronal pharmacological subtype. Researchers are interested in identifying the subtype of neuron that their tagged ES have matured into following introduction and integration into the recipient CNS. The kit includes antibodies for the identification of Glutamate neurons (EAAC1; AB1520-25UL), GABAergic neurons (GAD67; MAB5406-50UG), Dopaminergic neurons (TH; MAB5280-20UG), and serotonergic neurons (Serotonin; MAB352-50UL).
(2) Identify and quantify excitatory and inhibitory synapses involving the transplanted cells. In order to measure the level of functional integration of the tagged ES cells, researchers often use general markers of mature synaptic formation and identification of inhibitory GABAergic synaptic input onto the donor cells. The kit includes antibodies for the general identification of synapses (PSD-95; MAB1596-50UG) and (Synaptophysin; MAB5258-20UG) as well as for GABAergic synapses (GABAA Receptor b-chain; MAB341-50UG).
(1) Classify neuronal pharmacological subtype. Researchers are interested in identifying the subtype of neuron that their tagged ES have matured into following introduction and integration into the recipient CNS. The kit includes antibodies for the identification of Glutamate neurons (EAAC1; AB1520-25UL), GABAergic neurons (GAD67; MAB5406-50UG), Dopaminergic neurons (TH; MAB5280-20UG), and serotonergic neurons (Serotonin; MAB352-50UL).
(2) Identify and quantify excitatory and inhibitory synapses involving the transplanted cells. In order to measure the level of functional integration of the tagged ES cells, researchers often use general markers of mature synaptic formation and identification of inhibitory GABAergic synaptic input onto the donor cells. The kit includes antibodies for the general identification of synapses (PSD-95; MAB1596-50UG) and (Synaptophysin; MAB5258-20UG) as well as for GABAergic synapses (GABAA Receptor b-chain; MAB341-50UG).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Stem Cell Workflow Stage:
Characterization
Components:
- 50 µg of GABAA Receptor β-chain monoclonal antibody MAB341-50UG
- 50 µg of GAD67 monoclonal antibody MAB5406-50UG
- 50 µg of PSD-95 monoclonal antibody MAB1596-50UG
- 50 µL of Serotonin monoclonal antibody MAB352-50UL
- 20 µg of Synaptophysin monoclonal antibody MAB5258-20UG
- 20 µg of Tyrosine Hydroxylase monoclonal antibody MAB5280-20UG
- 25 µL of Glutamate Transporter, Neuronal (EAAC1) polyclonal antibody AB1520-25UL
Kit Type:
Neurobiology Kits
Gene Symbol:
- GABRB2
- MGC119386
- GABA
- MGC119389
- MGC119388
Stem Cell Type:
Neural Stem Cells
Kit or Assay Type:
- Neurobiology Kits
- Stem Cell Kits
Storage Conditions:
Maintain components according to the individual data sheets.