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Dopaminergic Neuron Integration and Characterization Kit
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | IH, IC | null | null | null |
Description:
Dopaminergic Neuron Integration and Characterization Kit
Trade Name:
Chemicon (Millipore)
Product Overview:
See individual data sheets for specific component descriptions.
Background Information:
Dopaminergic neuron dysfunction and loss correlates with numerous progressive and devastating neurological diseases. Stem cell research and applications are rapidly moving toward the introduction and subsequent post-development analysis of embryonic stem (ES) cell-derived dopaminergic neurons in adult tissue. The NS145 is part of an expanding Chemicon/Upstate product line targeting development and plasticity in neurobiology. NS145 is a novel, broadly encompassing kit for the localization, characterization and analysis of ES cell derived dopaminergic neuron integration into adult central nervous system (CNS) tissue. This kit allows researchers to: (1) identify and characterize dopaminergic neurons in tissue; and (2) identify other neighboring neuronal types and astrocytes. In addition, the antibodies are a mixture of mouse and rat monoclonals and rabit and goat polyclonals to optimize double, triple, and quadruple staining paradigms. The kit component breakdown is as follows:
(1) Identifying dopaminergic neurons. Researchers are interested in identifying the subtype of neuron that their tagged ES have matured into following introduction and integration into the recipient CNS. This is a key step in dopaminergic neuron replacement therapy. This kit includes antibodies for the identification of dopaminergic neurons using several diverse markers targeting different stages in the neurotransmitter synthesis and maintainance: Dopa Decarboxylase (AB1569-50UL); Tyrosine Hydroxylase (TH; MAB5280-20UG); Dopamine Transporter (DAT; MAB369-50UL).
(2) Identify other neuronal subtypes and astrocytes neighboring the transplanted cells. In order to measure the level of structural integration of the tagged ES cells, researchers often use general markers of neuronal subtype and astrocytes to characterize the local environment in which the ES cell-derived dopaminergic neurons are integrating. The kit includes antibodies for the general identification of neurons (NeuN; MAB377-100UG) and astrocytes (GFAP; AB5804). Also included are excellent markers for the identification of cholinergic (ChAT; AB144P-200UL) and Serotonergic (Serotonin; MAB352-50UL) neurons.
Additional, individual reagents can be ordered separately in larger quantities.
(1) Identifying dopaminergic neurons. Researchers are interested in identifying the subtype of neuron that their tagged ES have matured into following introduction and integration into the recipient CNS. This is a key step in dopaminergic neuron replacement therapy. This kit includes antibodies for the identification of dopaminergic neurons using several diverse markers targeting different stages in the neurotransmitter synthesis and maintainance: Dopa Decarboxylase (AB1569-50UL); Tyrosine Hydroxylase (TH; MAB5280-20UG); Dopamine Transporter (DAT; MAB369-50UL).
(2) Identify other neuronal subtypes and astrocytes neighboring the transplanted cells. In order to measure the level of structural integration of the tagged ES cells, researchers often use general markers of neuronal subtype and astrocytes to characterize the local environment in which the ES cell-derived dopaminergic neurons are integrating. The kit includes antibodies for the general identification of neurons (NeuN; MAB377-100UG) and astrocytes (GFAP; AB5804). Also included are excellent markers for the identification of cholinergic (ChAT; AB144P-200UL) and Serotonergic (Serotonin; MAB352-50UL) neurons.
Additional, individual reagents can be ordered separately in larger quantities.
Species Reactivity:
Vertebrates
Storage Conditions:
Maintain components according to the individual data sheets.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a table-top centrifuge to dislodge any liquid in the container's cap.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a table-top centrifuge to dislodge any liquid in the container's cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- DDC
- AADC
- EC 4.1.1.28
Components:
- One kit containing the following components:
- 100 μg of NeuN mouse monoclonal MAB377-100UG
- 50 μL of Dopa Decarboxylase rabbit polyclonal AB1569-50UL
- 50 μL of DAT rat monoclonal MAB369-50UL
- 50 μL of GFAP rabbit polyclonal AB5804
- 200 μL of ChAT goat polyclonal AB144P-200UL
- 50 μL of Serotonin rat monoclonal MAB352-50UL
- 20 μg of Tyrosine Hydroxylase mouse monoclonal MAB5280-20UG
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The dopamine transporter (DAT1) mediates the active reuptake of dopamine from the synapse and is a principal regulator of dopaminergic neurotransmission. The DAT1 gene has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse (Vandenbergh et al., 1992 [PubMed 1359373]).[supplied by OMIM]
Key Applications:
- Immunohistochemistry
- Immunocytochemistry
UniProt Summary:
FUNCTION: SwissProt: Q01959 # Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
SIZE: 620 amino acids; 68495 Da
SUBUNIT: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: Q01959 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
SIZE: 620 amino acids; 68495 Da
SUBUNIT: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: Q01959 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
Qty/Pk:
1 kit
Brand Family:
Chemicon
Product Name:
Dopaminergic Neuron Integration and Characterization Kit