Pellicon XL is a small volume Tangential Flow Filter (TFF) for pharmaceutical and biotechnology development applications. The Labscale TFF System accepts direct docking of the device, which eliminates the need for tubing connections.
True linear scalability with UF membranes with the same flow path and channel height as larger Pellicon 2 cassettes. The Pellicon XL 50 allows process development and scaling studies.
Can I use TFF systems to separate two proteins based on a significant difference in their respective molecular weights?
» Answer
As a general rule ultrafiltration does not provide high efficiency separations of protein mixtures. However, it may be possible to effect this type of separation between two proteins that have at least 10 X difference in molecular weight and if there is an ultrafiltration membrane available with a NMWL rating between the molecular weight of the two proteins. These separations are also generally more successful with dilute protein solutions and if run at low pressure.
How many times can I reuse TFF membranes?
» Answer
It is possible to reuse both ultrafiltration and microporous membranes used in Millipore Tangential flow systems (Miniplate, Prep Scale etc.). The number of uses for any given set of membranes is directly related to the effectiveness of the cleaning and storage protocols used. Please refer to the TFF Maintenance Guides for suggested cleaning and storage procedures.
Can the Labscale TFF System accommodate more than one Pellicon XL?
» Answer
PRODUCT: Multimanifold Kit for Labscale TFF System Catalogue No. Description US List Price XXMULTIMN Labscale Multimanifold Kit, 1/pk $150 DESCRIPTION: The Labscale Multimanifold Kit is designed to accommodate up to 3 Pellicon XL devices and mount directly onto the Labscale Reservoir. It may also be used with a peristaltic pump for those customers without a Labscale Reservoir. It is intended to be used for either membrane selection by choosing devices with different membranes or to increase surface area by choosing devices with the same membrane. The Labscale Multi-Manifold Kit includes 2 multi-port manifolds with assorted tubing fittings. Manifold fittings include male and female luer lock. Assorted fittings included male and female luer plugs, male to female luer lock elbows, and male luer lock to 1/8" barb. SPECIFICATIONS: Materials of Construction Manifold/Fittings/Connectors Polypropylene Tubing Tygon LFL Physical Characteristics Multi-Manifold with Packaging Length: 2.6 inches 6.6 inches Width: 2.8 inches 4.3 inches Weight: 0.2 lbs 0.34 lbs Fittings Male / Female Luer Lock Maximum Operating Pressure 80 psi