MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel, RCYTOMAG-80K

MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel, RCYTOMAG-80K

MILLIPLEX

96-well plate

To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:

• Performance equivalent to non-magnetic beads
• Improved performance with samples having high nonspecific binding
• More flexible plate / plate washer option

Therefore, the MILLIPLEX MAP Rat Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

Multiplexing

This is a two-hour or overnight incubation assay requiring 25 μL of 1:5 dilution serum/plasma samples. However, for best results an overnight incubation is recommended.

Rat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Design your multiplex kit by choosing from 23 analytes.

• 2 - 29 pg/ml
• Refer to kit protocol for sensitivites for individual cytokines/chemokines

Cytokines/Chemokines

• Inter-Assay: 6 - 14
• Intra-Assay: 3 - 16

80 - 109%

Luminex xMAP

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

MILLIPLEX MAP Rat Cytokine/Chemokine Magnetic Bead Panel, RCYTOMAG-80K

• 24 - 100,000 pg/mL for Rat Leptin
• 5 - 20,000 pg/mL for all other analytes

Eotaxin, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-12 (p70), IL-13, IL-17, IL-18, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IP-10, Leptin, MCP-1, MIP-1α, RANTES, TNF-α, VEGF