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Rat Gut Hormone Panel - 8 Plex
Description:
Rat Gut Hormone Panel - 8 Plex
Trade Name:
MILLIPLEX
Background Information:
Our understanding of obesity has been transformed by the identification of several gut hormones, peptide hormones secreted from the gastrointestinal tract. While much less in known about the specific role of these gut hormones in terms of the cause or initiation of obesity, we do know that these peptides play significant roles in the regulation of gut motility and secretion, pancreatic islet hormone secretion and food intake and energy expenditure. In addition evidence of their presence and synthesis may be detectable in other organs of the body, including the brain where they may function as neuropeptides, taking part in both neurotransmission and neuromodulation.
This Rat Gut Hormone multiplex assay simultaneously measures the hormones GLP-1 (active), GIP (total), Ghrelin (active), PYY (total), PP, Amylin (active), Leptin, and Insulin. This panel provides the researcher with a useful tool for studying the biological functions of these hormones in the gut, as well as determining the physiological implications these peptide hormones may have throughout the body. All components included in each kit are sufficient to run 38 samples in duplicate in a 96-well plate and have been validated for study in serum, plasma, and cell/tissue culture samples.
This Rat Gut Hormone multiplex assay simultaneously measures the hormones GLP-1 (active), GIP (total), Ghrelin (active), PYY (total), PP, Amylin (active), Leptin, and Insulin. This panel provides the researcher with a useful tool for studying the biological functions of these hormones in the gut, as well as determining the physiological implications these peptide hormones may have throughout the body. All components included in each kit are sufficient to run 38 samples in duplicate in a 96-well plate and have been validated for study in serum, plasma, and cell/tissue culture samples.
Key Applications:
Multiplexing
Application Notes:
25 µl sample volume Overnight assay
Species Reactivity:
Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Precision, %:
- Inter-assay: <24%
- Intra-assay: <7%
Sensitivity:
- Amylin(Active): 20 pg/ml
- Ghrelin(Active): 2 pg/ml
- GIP(Total): 1 pg/ml
- GLP-1(Active): 28 pg/ml
- Insulin: 28 pg/ml
- Leptin: 27 pg/ml
- PP: 17 pg/ml
- PYY (Total): 16 pg/ml
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Accuracy:
- Amylin(Active): 92%
- Ghrelin(Active): 90%
- GIP(total): 90%
- GLP-1(Active):: 84%
- Insulin: 93%
- Leptin: 87%
- PP: 88%
- PYY(total): 86%
Standard Curve Range:
- 3.0 - 2,000 pg/ml GIP
- 7.0 - 5,000 pg/ml Ghrelin, PP, PYY
- 41.0 - 30,000 pg/ml GLP-1, Amylin
- 69.0 - 50,000 pg/ml Insulin, Leptin
Kit Type:
Endocrine Kits
Assay Panel:
Customize your panel-order any or all of eight analytes
Configuration:
8 Plex: Select 8 Analytes
Analytes Available:
- Amylin (Active)
- Ghrelin (Active)
- GIP (Total)
- GLP-1 (Active)
- Insulin
- Leptin
- PP
- PYY (Total)
Detection Methods:
Luminex xMAP
Cross Reactivity:
Each antibody pair in a panel is specific and displays insignificant cross-reactivity to other analytes within the panel.
Kit or Assay Type:
Endocrine Kits