> Last Item Added:
0 Items

Ordering Information

  • : RI-13K
  • : 250 tubes

Rat Insulin RIA

Description:
Rat Insulin RIA
Qty/Pk:
250 tubes
Background Information:
Insulin, a 5.8 kD hormone is secreted by the islet beta cells in the pancreas.
Applications:
This radioimmunoassay (RIA) measures & quantifies Insulin for use in Metabolism & Endocrine research.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight, equilibrium incubation at 4º C. Sample volume 100 μL serum, plasma, or tissue culture media.
Species Reactivity:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Linearity of Dilution:
80 -100%
Label:
125I-Insulin
Product Name:
Rat Insulin RIA
Standard Curve Range:
0.1–10 ng/mL
Specificity:
Rat Insulin I 100% Rat Insulin II 100% Human Insulin 100% Human Proinsulin 69% Procine Insulin 100% Sheep Insulin 100% Hamster Insulin 100% Mouse Insulin 100% Rat C-Peptide Not Detectabl Glucagon Not Detectable Somatostatin Not Detectable Pancr
Analytes Available:
Insulin
UniProt Number:
Precision, %:
  • Inter-assay: 8.5–9.4
  • Intra-assay: 1.4–4.6
Detection Methods:
  • Radioactive
  • 125I
Accuracy:
85–93%
Performance:
ED80 = 0.2 ± 0.02 ng/mL
ED50 = 0.8 ± 0.04 ng/mL
ED20 = 3.0 ± 0.36 ng/mL

Product Resources