Subcellular Structure Identification Kit

Subcellular Structure Identification Kit

Chemicon (Millipore)

1 kit

Immunocytochemistry

Subcellular localization is a key functional characteristic of proteins. Restricting proteins within the cell determines their ability to interact with other proteins and small metabolites in their local environment. As recently as the late 1970's, the cell was often incorrectly described as a water-filled membranous sac enclosing a loosely structured population of discrete organelles. In reality, the interior of a cell is extremely compact, only a few times more open than a hydrated protein crystal [1]. The cell interior is crisscrossed by many tens of thousands of cytoskeletal filaments of various gauges, attaching organelles to the nucleus, plasma membrane, ECM and to each other [2].

CHEMICON®'s Subcellular Structure Localization Kit (Catalog number SC100) is a convenient and sensitive tool that allows for direct visualization of subcellular structures by fluorescence microscopy using antibodies and dyes.

The kit contains reagents for visualizing microfilament focal adhesion, golgi apparati, mitochondria, endoplasmic reticular actin cytoskeleton, and nuclei in cultured cells of human origin.

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• Mouse x Vinculin (Part No. 90319). One vial containing 100 μL of monoclonal antibody.
• Mouse x Golgi-Zone (Part No. 90320). One vial containing 100 μL of monoclonal antibody.
• Mouse x Mitochondria (Part No. 90321). One vial containing 100 μL of monoclonal antibody.
• ER stain (Part No. 90322). One vial containing 5 mg of endoplasmic reticulum stain powder.
• Goat x Mouse, IgG, FITC (Part No. 90323). One 10 mL bottle ready-to-use secondary antibody.
• Rhodamine-Phalloidin (Part No. 90324). One vial containing 15 μg of rhodamine-phalloidin.
• DAPI (Part No. 90325). One vial containing 100 mL of 0.1 mg/mL DAPI stain solution.

Chemicon

When stored at 2-8ºC, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Subcellular Structure Identification Kit

1. Tissue culture dishes and pipettes

2. Fixative (e.g. 4% paraformaldehyde)

3. Permeabilizing reagent (e.g. 0.1% Triton X-100)

4. 1 X Wash Buffer (e.g. TBST: 20mM Tris-HCl, pH 7.4, 0.15 NaCl, 0.05% Tween-20)

5. Blocking solution (e.g. 1% BSA in 1X PBS)

6. Alternative fluorescent-labeled secondary antibodies

7. Microscope

8. Glass slides and coverslips

9. Antifade mounting solution (Catalog No. 5013)