ReNcell CX Immortalized Cell Line
Chemicon (Millipore)
ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.
Stem Cell Culture
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Neural Stem Cells
Cells/Isolation
1. ReNcell NSC Maintenance Medium (CHEMICON Cat. No. SCM005)
2. ReNcell NSC Freezing Medium (CHEMICON Cat. No. SCM007)
3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity > 2 X 106 Units/mg. CHEMICON Cat. No. GF003)
4. Epidermal growth factor (EGF; Specific Activity > 1 x 107 Units/mg; CHEMICON Cat. No. GF001)
5. Laminin (Sigma Cat. No. L-2020)
6. DMEM/F12 w/o HEPES, w/ L-Glutamine (CHEMICON Cat. No. DF-042-B)
7. Accutase (CHEMICON Cat. No. SCR005)
8. Tissue culture-ware
9. Phosphate-Buffered Saline (1X PBS) (CHEMICON Cat. No. BSS-1005-B)
10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)
12. Primary and secondary antibodies 13.4'-6-Diamidino-2-phenylindole (DAPI) / PBS Solution
14. Anti-fading mounting solution (DABCO/PVA)
15. Hemacytometer
16. Microscope
> 1x106 viable ReNcell CX cells
Neural Stem Cells
Each lot of ReNcell CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.
When stored at the recommended storage conditions (liquid nitrogen), ReNcell CX cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
Human
Human cortical brain tissue
