ReNcell VM Human Neural Progenitor Cell LIne

ReNcell VM Human Neural Progenitor Cell LIne

Chemicon (Millipore)

ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell VM was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. In experiments performed by the ReNeuron Group plc, ReNcell VM can be differentiated in vitro to a high level of human dopaminergic neurons. Neurons differentiated from ReNcell VM have furthermore been shown to be electophysiologically active. ReNcell VM may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell VM cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.

Stem Cell Culture

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Human

Each lot of ReNcell VM cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities. Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Neural Stem Cells

Cells/Isolation

Neural Stem Cells

> 1x10⁶ viable ReNcell VM cells derived from human ventral mesencephalon brain tissue, cryopreserved. Store in liquid nitrogen.

Human ventral mesencephalon brain tissue

When stored at the recommended storage conditions (liquid nitrogen), ReNcell VM cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.

1. ReNcell NSC Maintenance Medium (CHEMICON Cat. No. SCM005)
2. ReNcell NSC Freezing Medium (CHEMICON Cat. No. SCM007)
3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity > 2 X 10⁶ Units/mg. CHEMICON Cat. No. GF003)
4. Epidermal growth factor (EGF; Specific Activity > 1 x 10⁷ Units/mg; CHEMICON Cat. No. GF001)
5. Laminin (Sigma Cat. No. L-2020)
6. DMEM/F12 w/o HEPES, w/ L-Glutamine (CHEMICON Cat. No. DF-042-B)
7. Accutase (CHEMICON Cat. No. SCR005)
8. Tissue culture-ware
9. Phosphate-Buffered Saline (1X PBS) (CHEMICON Cat. No. BSS-1005-B)
10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)
12. Primary and secondary antibodies
13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution
14. Anti-fading mounting solution (DABCO/PVA)
15. Hemacytometer
16. Microscope