FibroGRO™ Xeno-Free Human Foreskin Fibroblasts
Description:
FibroGRO™ Xeno-Free Human Foreskin Fibroblasts
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Millipore’s FibroGRO™ Xeno-Free Human Foreskin Fibroblasts are derived from normal human foreskin and have been isolated and manufactured under xeno-free conditions. Cells are supplied at a low passage number (p3 – p5) and proliferate rapidly when grown in FibroGROTM LS (low serum) Complete Medium (Cat. No. SCMF002). Rapid proliferation of human foreskin fibroblasts enable efficient reprogramming of the cells to induced pluripotent stem (iPS) cells. Using the lentivirus and protocol detailed in Millipore’s STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit (Cat. No. SCR530), reprogramming efficiency of 0.1% can be obtained with passage 6 FibroGRO™ Xeno-Free Human Foreskin Fibroblasts. Cells have been validated for reprogramming capacity and tested negative for mycoplasma. Human foreskin fibroblasts provide a useful cell line to aid in the dissection of the mechanism of reprogramming along with being a useful control to ascertain the reprogramming efficiencies of different delivery-based systems (i.e. adenovirus, lentivirus, small molecule, protein-based, episomal and minicircle DNA) or for use as a reprogramming control alongside a specific cell type. They can also be used to support feeder-dependent culture of human embryonic stem cells
Key Applications:
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Negative for mycoplasma, HTLV-1&2, Hepatitis B&C, HIV, Treponema palladium.
Stem Cell Workflow Stage:
Cells/Isolation
Components:
1 vial of Human Foreskin Fibroblasts
Brand Family:
Chemicon
Source:
Human foreskin fibroblasts, derived under xeno-free conditions
Storage Conditions:
Store in Liquid Nitrogen
Product Name:
FibroGRO™ Xeno-Free Human Foreskin Fibroblasts