ENStem-A™ Neural Expansion Medium
Description:
ENStem-A™ Neural Expansion Medium
Trade Name:
Chemicon (Millipore)
Product Overview:
ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.
Key Applications:
Stem Cell Culture
Application Notes:
Cell culture protocols for use with ENStem-A™ Human Neural Progenitor Cells are available in the datasheet that accompanies the ENStem-A™ Human Neural Progenitor Expansion Kit (Cat. No. SCR055).
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
Stem Cell Reagents
Media Form:
Liquid
Stem Cell Workflow Stage:
Maintenance & Expansion
Materials Required but Not Delivered:
• L-Glutamine (Cat. No. TMS-002-C)
• ENStem-A™ Human Neural Progenitor Expansion Kit (Cat. No. SCR055)
• ENStem-A™ Neural Freezing Medium (Cat. No. SCM011)
• ENStem-A™ Neuronal Differentiation Medium (Cat. No. SCM017)
• Poly-L-ornithine hydrobromide (Sigma Part No. P3655)
• Mouse Laminin (Sigma Part No. L-2020)
• Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C)
• Accutase™ (Cat. No. SCR005)
• Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B)
• Tissue culture-ware
• Hemacytometer
• Microscope
• ENStem-A™ Human Neural Progenitor Expansion Kit (Cat. No. SCR055)
• ENStem-A™ Neural Freezing Medium (Cat. No. SCM011)
• ENStem-A™ Neuronal Differentiation Medium (Cat. No. SCM017)
• Poly-L-ornithine hydrobromide (Sigma Part No. P3655)
• Mouse Laminin (Sigma Part No. L-2020)
• Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C)
• Accutase™ (Cat. No. SCR005)
• Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B)
• Tissue culture-ware
• Hemacytometer
• Microscope
Media Type:
Cell Culture Media
Formulation:
ENStem-ATM Neural Expansion Medium contains neurobasal medium, B27 supplement, and LIF.
Components:
- ENStem-A Neural Expansion Medium, 500 mL (Part No. SCM004a)
- FGF-2, 10 µg, lyophilized (Part No. GF003-10UG)
Stem Cell Type:
Neural Stem Cells
Quality Assurance:
Each lot of medium is tested for the optimal cell growth and proliferation of ENStem-ATM Human Neural Progenitor Cells.
Sterility Testing: Negative
Sterility Testing: Negative
Cell Culture Media or Reagent Type:
Stem Cell Culture Media
Storage Conditions:
ENStem-ATM Neural Expansion Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the expansion medium. Thawed should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.
FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C.
FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C.