ReNcell NSC Maintenance Media
Description:
ReNcell NSC Maintenance Media
Product Overview:
ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell immortalized neural progenitor cells
Key Applications:
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
- Cell Culture Supplements
- Stem Cell Reagents
Presentation:
ReNcell NSC Maintenance Medium contains DMEM/F12 w/o HEPES, w/ L-Glutamine (CHEMICON Cat. No. DF-042-B), L-glutamine (CHEMICON Cat. No. TMS-002-C), human serum albumin, human transferrin, putrescine dihydrochloride, human recombinant insulin, L-thyroxine, Tri-iodo-thyronine, progesterone, sodium selenite, heparin, corticosterone. The media does not contain antibiotics.
Media Form:
Liquid
Media Type:
Cell Culture Media
Materials Required but Not Delivered:
1. ReNcell CX Immortalized Cell Line (CHEMICON Cat. No. SCC007)
2. ReNcell VM Immortalized Cell Line (CHEMICON Cat. No. SCC008)
3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity > 2 X 106 Units/mg. CHEMICON Cat. No. GF003)
4. Epidermal growth factor (EGF; Specific Activity > 1 x 107 Units/mg; CHEMICON Cat. No. GF001) 5. Laminin (Sigma Cat. No. L-2020)
6. DMEM/F12 w/o HEPES, w/ L-Glutamine (CHEMICON Cat. No. DF-042-B)
7. Accutase (CHEMICON Cat. No. SCR005)
8. Tissue culture-ware
9. Phosphate-Buffered Saline (1X PBS) (CHEMICON Cat. No. BSS-1005-B)
10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
11. Blocking Solution (5 percent normal donkey serum, 0.3 % Triton X-100 in 1X PBS)
12. Primary and secondary antibodies
2. ReNcell VM Immortalized Cell Line (CHEMICON Cat. No. SCC008)
3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity > 2 X 106 Units/mg. CHEMICON Cat. No. GF003)
4. Epidermal growth factor (EGF; Specific Activity > 1 x 107 Units/mg; CHEMICON Cat. No. GF001) 5. Laminin (Sigma Cat. No. L-2020)
6. DMEM/F12 w/o HEPES, w/ L-Glutamine (CHEMICON Cat. No. DF-042-B)
7. Accutase (CHEMICON Cat. No. SCR005)
8. Tissue culture-ware
9. Phosphate-Buffered Saline (1X PBS) (CHEMICON Cat. No. BSS-1005-B)
10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
11. Blocking Solution (5 percent normal donkey serum, 0.3 % Triton X-100 in 1X PBS)
12. Primary and secondary antibodies
Storage Conditions:
SCM005, ReNcell Neural Stem Cell Maintenance Medium should be stored at -20°C until ready to use. Upon thawing, the maintenance medium should be stored at 2-8°C and given a 1-month expiration dating.