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Mouse Neural Stem Cell Expansion Medium
Description:
Mouse Neural Stem Cell Expansion Medium
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
The Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.
Key Applications:
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Each lot of medium is tested for the optimal cell growth, proliferation and differentiation of mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4
Cell Culture Media or Reagent Type:
Neural Stem Cell Culture Media
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- Differentiation
Media Type:
Cell Culture Media
Components:
- 500 mL of Neural Stem Cell Basal Medium (Cat.# SCM003)
- bFGF, 10 ug
- EGF, 10 ug
- Heparin, 1.5 mg
Brand Family:
Chemicon
Storage Conditions:
Part number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw.
bFGF, EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw.
bFGF, EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
Cell Culture Reagent Type:
Stem Cell Reagents
Product Name:
Mouse Neural Stem Cell Expansion Medium
Materials Required but Not Delivered:
• Cryopreserved Mouse Cortical Neural Stem Cells (Millipore Catalog No. SCR029)
• Cryopreserved Mouse Spinal Cord Neural Stem Cells (Millipore Catalog No. SCR031)
• Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
• Poly-L-ornithine (Sigma Catalog No. P3655)
• Laminin (Millipore Catalog No. CC095)
• Accutase solution (Millipore Catalog No. SCR005)
• Cryopreserved Mouse Spinal Cord Neural Stem Cells (Millipore Catalog No. SCR031)
• Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
• Poly-L-ornithine (Sigma Catalog No. P3655)
• Laminin (Millipore Catalog No. CC095)
• Accutase solution (Millipore Catalog No. SCR005)