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Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCR022) differentiated for 6-9 days in Astrocyte Differentiation Medium (Catalog No. SCM010). Ove...
ASTROCYTE DIFFERENTIATION MEDIUM
Description:
ASTROCYTE DIFFERENTIATION MEDIUM
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
The Astrocyte Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of rodent neural stem cells to an astrocyte lineage. The medium has been extensively validated on mouse cortical and spinal cord neural stem cells and on rat hippocampal neural stem cells. Using Millipore's Astrocyte Differentiation Medium, relatively pure populations of astrocytes (>80-90% GFAP) are obtained. Very low levels of neurons (<1% MAP2A,2B positive) or oligodendrocytes (<5% O1 positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.
Key Applications:
Cell Culture
Species Reactivity:
- Mouse
- Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Each lot of media is tested for the optimal differentiation of rodent neural stem cells to an astrocyte lineage.
Sterility Testing: Negative
Sterility Testing: Negative
Cell Culture Media or Reagent Type:
Neural Stem Cell Culture Media
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Differentiation
Media Type:
Cell Culture Media
Components:
- 100 mL of Astrocyte Differentiation Medium (Part No. SCM010A)
- 10 μg of Basic FGF-2, lyophilized (Part No. GF003-10UG)
Brand Family:
Chemicon
Presentation:
The Rat Astrocyte Differentiation Medium contains DMEM/F12 w/o HEPES, w/ L-Glutamine (Millipore Catalogue No. DF-042-B), 1X B27-CHEM, 2 mM L-Glutamine (Millipore Catalogue No. TMS-002-C), 1X Penicillin Streptomycin (Millipore Catalogue No. TMS-AB2-C), BMP-4, 50 ng/mL LIF (Millipore Catalogue No. ESG1107) and 1% heat-inactivated fetal bovine serum (Millipore Catalogue No. ES-009-B)
Storage Conditions:
SCM010A, Astrocyte Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C and given a 1-month expiration dating.
FGF-2 (10 μg, lyophilized) should be reconstituted with 100 μL 5mM Tris-HCl, pH 7.6 for a final concentration of 100 μg/mL. Dispense into aliquots to avoid repeated freeze/thaw.
FGF-2 (10 μg, lyophilized) should be reconstituted with 100 μL 5mM Tris-HCl, pH 7.6 for a final concentration of 100 μg/mL. Dispense into aliquots to avoid repeated freeze/thaw.
Product Name:
ASTROCYTE DIFFERENTIATION MEDIUM
Materials Required but Not Delivered:
• Rodent neural stem cells (Millipore Catalogue No. SCR022, SCR029, SCR031)
• Neural Stem Cell Characterization Kit (Millipore Catalogue No. SCR019)
• Neural Stem Cell Basal Medium (Millipore Catalogue No. SCM003)
• Rat/Mouse Neural Stem Cell Expansion Medium (Millipore Catalogue No. SCM008, SCM009)
• Poly-L-ornithine (Sigma Catalogue No. P3655)
• Laminin (Millipore Catalogue No. CC095)
• AccutaseTM (Millipore Catalogue No. SCR005)
• Hemocytometer
• EGF (> 107 units/mg; Millipore Catalogue No. GF001)
• Heparin (Sigma Catalogue No. H-1027)
• Neural Stem Cell Characterization Kit (Millipore Catalogue No. SCR019)
• Neural Stem Cell Basal Medium (Millipore Catalogue No. SCM003)
• Rat/Mouse Neural Stem Cell Expansion Medium (Millipore Catalogue No. SCM008, SCM009)
• Poly-L-ornithine (Sigma Catalogue No. P3655)
• Laminin (Millipore Catalogue No. CC095)
• AccutaseTM (Millipore Catalogue No. SCR005)
• Hemocytometer
• EGF (> 107 units/mg; Millipore Catalogue No. GF001)
• Heparin (Sigma Catalogue No. H-1027)