ENStem-A™ Neuronal Differentiation Medium
Description:
ENStem-A™ Neuronal Differentiation Medium
Trade Name:
Chemicon (Millipore)
Product Overview:
ENStem-A™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A™ human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A™ Human Neural Progenitor Cells. Using ENStem-A™ Neuronal Differentiation Medium, relatively pure populations of neuronal cells (>90% bIII-tubulin positive) are obtained. Very low levels of astrocytes (<0.5% GFAP positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.
Key Applications:
Stem Cell Culture
Application Notes:
Cell culture protocols for use with ENStem-A™ Human Neural Progenitor Cells are available in the datasheets that accompany the ENStem-A™ Human Neural Progenitor Expansion Kit (Part No. SCR055).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
Stem Cell Reagents
Media Form:
Liquid
Stem Cell Workflow Stage:
Differentiation
Materials Required but Not Delivered:
• L-Glutamine (Cat. No. TMS-002-C)
• ENStem-A™ Human Neural Progenitor Cells Kit (Cat. No. SCR055)
• ENStem-A™ Neural Expansion Medium (Cat. No. SCM004)
• ENStem-A™ Neural Freezing Medium (Cat. No. SCM011)
• Human Neural Stem Cell Characterization Kit (Cat. No. SCR060)
• Poly-L-ornithine (Sigma Part No. P3655)
• Mouse Laminin (Sigma Part No. L-2020)
• Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C)
• Accutase™ (Cat. No. SCR005)
• Chamber slides
• Tissue culture-ware
• Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B)
• Hemocytometer
• Microscope
• ENStem-A™ Human Neural Progenitor Cells Kit (Cat. No. SCR055)
• ENStem-A™ Neural Expansion Medium (Cat. No. SCM004)
• ENStem-A™ Neural Freezing Medium (Cat. No. SCM011)
• Human Neural Stem Cell Characterization Kit (Cat. No. SCR060)
• Poly-L-ornithine (Sigma Part No. P3655)
• Mouse Laminin (Sigma Part No. L-2020)
• Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C)
• Accutase™ (Cat. No. SCR005)
• Chamber slides
• Tissue culture-ware
• Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B)
• Hemocytometer
• Microscope
Media Type:
Stem Cell Culture Media
Stem Cell Type:
Neural Stem Cells
Quality Assurance:
Each lot of media is tested for the optimal differentiation of ENStem-A™ Human Neural Progenitor Cells to a neuronal lineage.
Sterility Testing: Negative
Sterility Testing: Negative
Cell Culture Media or Reagent Type:
Stem Cell Culture Media
Storage Conditions:
ENStem-A™ Neuronal Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the medium. Thawed medium should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.