Embryoid Body (EB) Formation Medium
Description:
Embryoid Body (EB) Formation Medium
Product Overview:
The Embryoid Body (EB) Formation Medium has been optimized and qualified to support the formation of embryoid body. The medium can be used to form EB from hanging drops or suspension culture on non-adhesive Petri dishes. It has been validated for the differentiation of mouse ES cells into neural, adipogenic and cardiomyogenic cell lineages.
Background Information:
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Most protocols used to differentiate murine ES cells to a variety of cells involve an initial step in which the spontaneous differentiation of ES cells proceed through the formation of embryoid body (EB). EBs are formed by culturing ES cells in suspension culture in low attachment Petri dishes. The non-adhesive surfaces of Petri dishes promote the aggregation of ES cells into a tight ball of cells termed embryoid body. EBs are thought to help maintain an embryonic organization and closely mimic some of the earliest stages of embryonic development.
Key Applications:
Cell Differentiation
Media Type:
Stem Cell Culture Media
Formulation:
Embryoid Body (EB) Formation Medium is a proprietary formulation that contains fetal bovine serum.
Storage Conditions:
Stored at -20°C until ready to use. Upon thawing, store at 2-8°C for up to one month.