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MilliTrace Rat Neural Stem Cell Expansion Medium
Description:
MilliTrace Rat Neural Stem Cell Expansion Medium
Qty/Pk:
1 kit
Product Overview:
MilliTrace Rat Neural Stem Cell Expansion Medium is provided as a multi-component system that has been optimized for use with the MilliTrace line of GFP reporter rat neural stem cells. The Neural Stem Cell Basal Medium is a defined serum-free medium that helps support the growth and in vitro differentiation of neural stem cells derived from rodents. Basic Fibroblast Growth Factor (FGF-2, bFGF) is provided separately, which when added to the Neural Stem Cell Basal Medium (SCM003) allows for the growth and proliferation of rat neural stem cells. Withdrawal of FGF-2 from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rat neural stem cells. Puromycin Solution is provided separately to help maintain the expression of the GFP labeled transgene.
Key Applications:
Stem Cell Culture
Cell Culture Media or Reagent Type:
Neural Stem Cell Culture Media
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Media Type:
Cell Culture Media
Components:
- Neural Stem Cell Basal Medium, 500 mL
- Basic FGF, 10 µg
- Puromycin Solution, 100 µL
Storage Conditions:
· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
· Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
· 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.
· Basic FGF (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
· 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.
Product Name:
MilliTrace Rat Neural Stem Cell Expansion Medium
Materials Required but Not Delivered:
· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit (Millipore Catalog No. SCR080)
· Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
· Poly-L-ornithine (Sigma Catalog No. P3655)
· Laminin (Millipore Catalog No. CC095)
· Accutase™ (Millipore Catalog No. SCR005)
· Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
· Poly-L-ornithine (Sigma Catalog No. P3655)
· Laminin (Millipore Catalog No. CC095)
· Accutase™ (Millipore Catalog No. SCR005)