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MilliTrace Rodent Neural Stem Cell Basal Medium
Description:
MilliTrace Rodent Neural Stem Cell Basal Medium
Qty/Pk:
1 kit
Product Overview:
MilliTrace Rodent Neural Stem Cell Basal Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of MilliTrace GFP reporter neural stem cells derived from rodents. When used in conjunction with puromycin (provided) and FGF or FGF, EGF and heparin (not provided), the basal medium will allow for the proliferation of MilliTrace GFP reporter rat and mouse neural stem cells, respectively. Removal of the growth factors from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of MilliTrace GFP reporter rodent neural stem cells. Puromycin Solution is provided to maintain the expression of the transgene.
Key Applications:
Stem Cell Culture
Cell Culture Media or Reagent Type:
Neural Stem Cell Culture Media
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Media Type:
Cell Culture Media
Components:
- Neural Stem Cell Basal Medium, 500 mL
- Puromycin Solution, 100 µL
Storage Conditions:
· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
· 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.
· 5 mg/mL Puromycin Solution, 100 µL (Part No. CS201361) should be stored in working aliquots at -20°C for up to two years.
Product Name:
MilliTrace Rodent Neural Stem Cell Basal Medium
Materials Required but Not Delivered:
· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit (Millipore Catalog No. SCR080)
· MilliTrace Constitutive GFP Mouse Cortical Neural Stem Cell Kit (Millipore Catalog No. SCR081)
· Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
· Poly-L-ornithine (Sigma Catalog No. P3655)
· Laminin (Millipore Catalog No. CC095)
· Accutase™ (Millipore Catalog No. SCR005)
· MilliTrace Constitutive GFP Mouse Cortical Neural Stem Cell Kit (Millipore Catalog No. SCR081)
· Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
· Poly-L-ornithine (Sigma Catalog No. P3655)
· Laminin (Millipore Catalog No. CC095)
· Accutase™ (Millipore Catalog No. SCR005)