FibroGRO Complete Medium is a defined medium optimized for serum-free culture of human fibroblasts, and supports the growth of these cells in a serum-free environment at rates equal to or greater than comparable media supplemented with much higher concentrations of FBS. FibroGRO Medium contains no antimicrobials and no phenol red; components that can cause cell stress and “masking effects” that may influence experimental results (these are not needed, or recommended, to achieve optimal cell performance). FibroGRO medium provides a perfect cell system to establish serum-free human feeder layers for stem cell culture, or as a model to study wound healing, toxicology or basic cell biology