Mesenchymal Stem Cell Characterization Kit

Mesenchymal Stem Cell Characterization Kit

Chemicon (Millipore)

1 kit

Millipore's Mesenchymal Stem Cell Characterization Kit (Catalog Number SCR018) contains a panel of positive and negative selection markers for the characterization of the mesenchymal stem cell population in rat. Positive cell markers include antibodies directed against cell-surface molecules (integrin beta1 and CD54) (Pittenger, M. F., and Marshak, D. R., & Pittenger, M.F., et al.) present on mesenchymal stem cells along with two extracellular matrix molecules (fibronectin and type I collagen) that are synthesized by cultured mesenchymal stem cells (Prockop, D. J.). Along with the positive selection markers, two specific hematopoietic cell surface markers, (CD14 - present on leukocytes and CD45 - present on monocytes and macrophages) are provided whose expressions should not be present on mesenchymal stem cells (Pittenger, M. F., and Marshak, D. R., & Alhadlaq, A., and Mao J. J, & Pittenger, M.F.,et al.). Mouse and rabbit immunoglobulins for the assessment of background staining are also included.

For Research Use Only; not for use in diagnostic procedure.

• Immunocytochemistry
• Stem Cell Culture

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• Pancreatic Stem Cells
• Mesenchymal Stem Cells

Characterization

• Rabbit anti-Integrin β1 (Catalog No. AB1952-20): Known species reactivity: Hu, Ms, Rt, other species not tested. One vial containing 20 µL polyclonal antibody.
• Mouse anti-CD54 (ICAM-1)(Catalog No. MAB2130, clone W-CAM-1): Known species reactivity: Hu., other species not tested. One vial containing 100 µL monoclonal antibody.
• Rabbit anti-Collagen Type I (Catalog No. 2003605): Known species reactivity: Rt, other species not tested. One vial containing 20 µL polyclonal antibody.
• Rabbit anti-Fibronectin (Catalog No. 2003606): Known species reactivity: Rt, other species not tested. One vial containing 10 µg polyclonal antibody.
• Mouse anti-CD45 (Catalog No. 2003607, clone OX-1): Known species reactivity: Rt, other species not tested. One vial containing 100 µL monoclonal antibody.
• Mouse anti CD14 (Catalog No. 2003608, clone UCHM-1): Known species reactivity: Hu, Mky, other species not tested. One vial containing 10 µL monoclonal antibody.
• Mouse IgG (Catalog No. PP54-100UG): One vial containing 100 µg purified mouse IgG control antibody.
• Rabbit IgG (Catalog No. PP64-100UG): One vial containing 100 µg purified rabbit IgG antibody.

Chemicon

When stored at -20°C, components are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Mesenchymal Stem Cell Characterization Kit

1. Mesenchymal stem cells and culture reagents (Cat. No. SCR027).
2. Mesenchymal Stem Cell Expansion Media (Cat. No. SCM015)
3. Chamber slides
4. Glass coverslips
5. Phosphate-Buffered Saline (1X PBS)
6. Fixative (e.g. freshly made 4% Paraformaldehyde in 1X PBS, pH 7.4)
7. Blocking Solution (5% normal donkey serum, with 0.3% Triton X-100 in 1X PBS filtered through a 0.45µm filter)
8. Non-Permeable Blocking Solution (5% normal donkey serum in 1X PBS, filtered through a 0.45µm filter)
9. Fluorescent-labeled secondary antibodies. Donkey anti-mouse IgG, Cy3 conjugated (Cat. No. AP192C) and donkey anti-rabbit IgG, Cy3 conjugated (Cat. No. AP182C) are recommended
10. Antibody dilution buffer (1X PBS without blocking or detergents).
11. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution (200ng/mL in PBS). Note keep solution out of light.
12. Anti-fading mounting solution (Cat. No. 5013).
13. Fluorescent Microscope