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- : SCR023
- : 1 kit
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Endothelial Cell Characterization Kit
Description:
Endothelial Cell Characterization Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Endothelial Cell Characterization Kit
Antibodies for the immunocytochemical characterization of endothelial cells.
Antibodies for the immunocytochemical characterization of endothelial cells.
Background Information:
Endothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction and vasodilation. Chemicon's endothelial cell characterization kit enables researchers to characterize cultured endothelial cells in both a constitutive and activated state using basic immunocytochemistry techniques.
Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry:
* Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL
* Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL
* Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL
The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions:
* Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL
* Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml
Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry:
* Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL
* Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL
* Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL
The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions:
* Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL
* Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml
Key Applications:
Immunocytochemistry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Type:
- Mouse Embryonic Stem Cells
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Cardiac Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Characterization
Components:
- 50 μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)
- 50 μg of CD31 monoclonal (Part No. 2003788)
- 10 μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)
- 50 μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)
- 50 μg of ICAM-1 monoclonal (Part No. 2003785)
- 100 μg of Mouse control IgG (Part No. PP54-100UG)
- 100 μg of Rabbit control IgG (Part No. PP64-100UG)
Brand Family:
Chemicon
Storage Conditions:
Antibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2 - 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).
Product Name:
Endothelial Cell Characterization Kit