Cryopreserved Mouse Cortical Neural Stem Cells

Description:

Trade Name:

Chemicon (Millipore)

Qty/Pk:

1 vial

Product Overview:

Millipore's Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.

We recommend that Millipore's Cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population.

For Research Use Only; not for use in diagnostic procedure.

Key Applications:

Stem Cell Culture

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Species:

Mouse

Stem Cell Type:

Neural Stem Cells

Stem Cell Workflow Stage:

Cells/Isolation

Cell Line Type:

Neural Stem Cells

Components:

1x10e6 viable Mouse Cortical Neural Stem Cells: (Part No. 2004115) derived from mixed gender C57/BL6 embryonic day 15-18 mice, cryopreserved. Store in liquid nitrogen.

Brand Family:

Chemicon

Storage Conditions:

When stored at the recommended storage conditions (liquid nitrogen), mouse cortical neural stem cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.

Packaging:

1 x 10⁶ cells

Product Name:

Cryopreserved Mouse Cortical Neural Stem Cells

Materials Required but Not Delivered:

1. Neural Stem Cell Expansion Medium (Millipore Catalog No. SCM003)

2. Basic fibroblast growth factor (bFGF, FGF-2; Specific Activity

> 2 x 10e6 Units/mg, Millipore Catalog No. GF003)

3. Epidermal Growth Factor (EGF; Specific Activity > 1 x 10e7 Units/mg; Millipore Catalog No. GF001)

4. Heparin (Sigma Catalog No. H-1027)

5. Poly-L-ornithine (Sigma Catalog No. P3655)

6. Laminin (Millipore Catalog No. CC095)

7. ESGRO Complete™ Accutase™ (Millipore Catalog No. SF006)

8. Tissue culture-ware

9. Phosphate-Buffered Saline (1X PBS) (Millipore Catalog No. BSS-1005-B)

10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)

11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)

12. Primary and secondary antibodies

13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution

14. Anti-fading mounting solution (DABCO/PVA)

15. Hemacytometer

16. Microscope

Ordering Information

Cryopreserved Mouse Cortical Neural Stem Cells

Product Resources

Brochures

User Guides

Product Support