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Cryopreserved Mouse Cortical Neural Stem Cells
Description:
Cryopreserved Mouse Cortical Neural Stem Cells
Trade Name:
Chemicon (Millipore)
Product Overview:
Introduction:
Neural stem cells (NSC) are self-renewing cells with the capacity to differentiate into neurons, astrocytes and oligodendrocytes. Due to their therapeutic promise, considerable attention has been focused on identifying the sources of stem cells, the signals that regulate their proliferation and the specification of neural stem cells towards more differentiated cell lineages.
Presently, neural stem cells are often identified based upon the presence of molecular markers that correlate with the stem and/or progenitor state along with the absence of differentiated phenotypes as assessed through marker analysis. To aid researchers in the accurate identification and characterization of neural stem cells, CHEMICON® presents cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029).
CHEMICON®'s Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.
We recommend that CHEMICON®'s Cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population. Please look for these differentiation assays from CHEMICON? in the near future.
For Research Use Only; not for use in diagnostic procedure.
Neural stem cells (NSC) are self-renewing cells with the capacity to differentiate into neurons, astrocytes and oligodendrocytes. Due to their therapeutic promise, considerable attention has been focused on identifying the sources of stem cells, the signals that regulate their proliferation and the specification of neural stem cells towards more differentiated cell lineages.
Presently, neural stem cells are often identified based upon the presence of molecular markers that correlate with the stem and/or progenitor state along with the absence of differentiated phenotypes as assessed through marker analysis. To aid researchers in the accurate identification and characterization of neural stem cells, CHEMICON® presents cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029).
CHEMICON®'s Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.
We recommend that CHEMICON®'s Cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population. Please look for these differentiation assays from CHEMICON? in the near future.
For Research Use Only; not for use in diagnostic procedure.
Key Applications:
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Mouse
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Cells/Isolation
Cell Line Type:
Neural Stem Cells
Components:
1x10e6 viable Mouse Cortical Neural Stem Cells: (Part No. 2004115) derived from C57/BL6 embryonic day 15-18 mice, cryopreserved. Store in liquid nitrogen.
Storage Conditions:
When stored at the recommended storage conditions (liquid nitrogen), mouse cortical neural stem cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
Packaging:
1 x 106 cells
Materials Required but Not Delivered:
1. Neural Stem Cell Expansion Medium (Chemicon Catalog No. SCM003)
2. Basic fibroblast growth factor (bFGF, FGF-2; Specific Activity
> 2 x 10e6 Units/mg. CHEMICON® Catalog No. GF003)
3. Epidermal Growth Factor (EGF; Specific Activity > 1 x 10e7 Units/mg; Catalog No. GF001)
4. Heparin (Sigma Catalog No. H-1027)
5. Poly-L-ornithine (Sigma Catalog No. P3655)
6. Laminin (Sigma Catalog No. L-2020)
7. ESGRO Complete™ Accutase™ (CHEMICON® Catalog No. SF006)
8. Tissue culture-ware
9. Phosphate-Buffered Saline (1X PBS) (CHEMICON® Catalog No. BSS-1005-B)
10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)
12. Primary and secondary antibodies
13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution
14. Anti-fading mounting solution (DABCO/PVA)
15. Hemacytometer
16. Microscope
2. Basic fibroblast growth factor (bFGF, FGF-2; Specific Activity
> 2 x 10e6 Units/mg. CHEMICON® Catalog No. GF003)
3. Epidermal Growth Factor (EGF; Specific Activity > 1 x 10e7 Units/mg; Catalog No. GF001)
4. Heparin (Sigma Catalog No. H-1027)
5. Poly-L-ornithine (Sigma Catalog No. P3655)
6. Laminin (Sigma Catalog No. L-2020)
7. ESGRO Complete™ Accutase™ (CHEMICON® Catalog No. SF006)
8. Tissue culture-ware
9. Phosphate-Buffered Saline (1X PBS) (CHEMICON® Catalog No. BSS-1005-B)
10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)
12. Primary and secondary antibodies
13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution
14. Anti-fading mounting solution (DABCO/PVA)
15. Hemacytometer
16. Microscope